Comparison of human and porcine skins as the barrier membraneinan in vitro permeation of plant polyphenols
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Abstract
Introduction: Porcine and human skin were compared as barrier membranes in in vitro permeation of polyphenols from plant extracts as sources of antioxidants to be used as value-added ingredients in novel cosmetic products. In vitro permeation was intended to assess expected release of ingredients from the applied products. Materials and Method: Excised skins from porcine ears and human abdomen were used as the barrier membrane in diffusion cells for comparison of polyphenols from 7 plant extracts; dahla (EE), damask rose(RD), buaput (RK), samohtai (TC), samohpipek (TB), blue butterfy pea (CT) and red rose (RH) at 32 oC. Total polyphenols were analyzed by Folin-Ciocalteaucolorimetry at 765 nm using gallic acid as the standard and permeation rates were statistically compared. Results: Donor concentrations of EE, RD, RK, TC, TB, CT and RH were 5, 13.5, 56, 42, 37, 3.6 and 8.9 mg GAE·ml-1. Relationships between donor polyphenol concentrations and permeation rates using porcine skin showed a linear correlation (r = 0.826) but not when using the human skin as the barrier (r = 0.321). There were lag time of about 12 – 22 h when using the human skin but not porcine. Thus, porcine and the human skin did not provide the same permeation profles which included rate, cumulative amount and lag time. Conclusion: Porcine skin cannot be used as a substitute of the human skin in in vitro permeation of plant extracts with substantially low quantities of polyphenols.
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