Application of HPLC Method for Determination of Caffeine in Diet 3 in 1 Instant Coffee Products Commercially Sold in KhonKaen Province

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Supawadee Daodee
Yaowares Chulikit
Duangsuda Apiwatdamrongkit


Introduction: Caffeine  is  one  of  the  constituent which  generally  found  in Diet  3  in  1  instant  coffee products. These products were commercially sold in Muang District, KhonKaenProvince so the aim of this study was to determine the amount of caffeine in these products. Materials and Methods: A simple HPLC method was applied to determine the amount of caffeine in these products. Liquid-liquid extraction was used for the extraction of caffeine from these samples. HPLC separation was carried out using Hypersil ODS C18 column and detected at wavelength 254 nm. This chromatographic condition using the mixture of methanol and water with constant ratio at 65:35 was found to complete resolution of caffeine. This HPLC method was validated in the topics of specifcity, accuracy, precision, linearity, limit of detection and limit of quantitation and could give reliable results. Results: From the result of this study, the amount of caffeine in these products was found from 37 mg to 107 mg per pack which can cause health problem for over consumption of these products. Conclusion: Therefore, the awareness of consumption these products should be provided for the people who would like to use these products for the diet and weight control purpose to protect from the adverse side effect from caffeine overdose.


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