Characterization of Newcastle Disease Virus Propagated from LLC MK2 Cell Culture

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Ratiya Kukhetpitakwong
Preecha Homchampa
Jarunee Satra
Thitawat Chantaworn
Sarisa Trakarnrungsee
Chariya Hahnvajanawong
Vichai Leelavacharamas
Watcharee Khunkitti

Abstract

Introduction: Newcastle disease virus (NDV) strain LaSota is commonly used as a vaccine strain for live vaccines. The virus is commonly cultivated from specific pathogen-free embryonated eggs. Propagation of the virus in cell culture is an alternative for vaccine production. The objective of this study was to investigate the differentiation of NDV propagated from LLC MK2 cell culture. Methods: Reverse Transcription-Polymerase Chain Reaction (RT-PCR) was used to prepare the fusion (F) gene of the adapted virus. Splenocyte proliferation and specific antibody response in immunized BALB/c mice were studied. Results and Discussion: The F gene of the adapted virus was still of lentogenic strain. Two-point mutations on the DNA sequences of the virus were observed without a change in amino acids. The virus could stimulate splenocyte proliferation and induce an increase of serumIgG2a and IgG2b levels similar to those of the NDV from embryonated eggs. Conclusion: The adapted virus from LLC MK2 cells could be used as antigen for a live vaccine.

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Article Details

Section
Pharmaceutical Sciences
Author Biographies

Jarunee Satra, eterinary Biologics Assay Center, Bureau of Veterinary Biologics, Department of Livestock Development, Ministry of Agricultureand Cooperatives, Pakchong, Nakornratchasima 30310 THAILAND

Veterinary Biologics Assay Center, Bureau of Veterinary Biologics, Department of Livestock Development, Ministry of Agricultureand Cooperatives, Pakchong, Nakornratchasima 30310 THAILAND

Watcharee Khunkitti, Faculty of Pharmaceutical Sciences, Khon Kaen Unversity

Faculty of Pharmaceutical Sciences, Khon Kaen Unversity

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