Development of Intramammary Dry Powder for Suspension in Dairy Cows Use
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Abstract
Mastitis in dairy cows has been a major economic problem for dairy industries. Nowadays, the main problem of intramammary preparations is insufficient amounts of drugs at the target site. It is due to the fact that the preparations do not spread and adhere in udders. Objective: This research was aimed to develop formulations by reducing particle sizes of the preparations to increase spreadability in udder. Method: Clindamycin HCl was selected as a model drug to develop suspensions. Chitosan (cationic charge), sodium carboxymethylcellulose; NaCMC (anionic charge) and hydroxypropyl methylcellulose; HPMC (nonionic charge) were chosen to develop the preparations in this study. The proportions of each polymer and clindamycin HCl were varied as 0.5:1, 1:1 and 2:1. All formulas were generated as dried powders via spray drying process. Result: There were only the formulas which contained NaCMC and HPMC 4000 in the ratio of 1:1 and 2:1 gave the percentages of entrapment in the ranges of 90.00-120.00%. The morphology was spherical and particle sizes were in the ranges of microns. Those formulas were selected to evaluate in vitro release study in phosphate buffered (pH 7.4). The results showed that the formulas which were composed of NaCMC or HPMC 4000 in the ratio of 1:1 were completely released within 30 minutes. Therefore, those formulas were carried out in vitro release study in full cream milk. The results showed that only NaCMC formula gave the percentages of clindamycin HCl released at 75.06%, that was close to the criteria (Q≥85%). The NaCMC formula could inhibit Staphylococcus aureus. Conclusion: The NaCMC (1:1) formula gave a good trend to develop in the further experiments.
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