Inhibitory effects of polyphenol from mangosteen extracts on Streptococcus mutans in vitro
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Abstract
Introduction: Dental caries is an important dental problem. Streptococcus mutans (s. mutans) is a leading cause of dental caries and associated with caries development. Previous studies have shown that the extracts from pericarp of mangosteen have demonstrated antibacterial activity against a wide variety of microorganisms. Phytochemical studies have shown that its active components are polyphenolic compounds. Among these, Q-mangostin has the most potent antibacterial activity. The aim of this study was to examine the antibacterial activity of polyphenol from mangosteen extracts against s. mutans. Materials and methods: Chemical pattern of the 95% ethanol extract of mangosteens were investigated by TLC. The total phenolic contents were determined by Folin-Ciocalteu Colorimetric Method. The antibacterial activity of s. mutans ATCC 25175 was determined by broth dilution method (2-fold serial dilution) in microtiter 96-well plate and drop plate. Antibacterial activity was expressed as minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Results: The mangosteen extracts composed of Q-mangostin as one of chemical constituents. The total phenolic contents of mangosteen extracts was 25.88 pg/mL gallic acid equivalence (GAE). The extracts was effective against ร. mutans ATCC 25175. The MIC was 0.25 mg/mL and the MBC was 1 mg/mL. Conclusion: The results showed the antibacterial activity of polyphenol from mangosteen extracts against S. mutans, the leading cause of dental caries and associated with caries development.
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