Antioxidant and Antityrosinase activities of silk sericin from silkworm UB1 X UB5 comparison with commercial products
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Abstract
Introduction: Silk sericin is a kind of water-soluble globular protein derived from silkworm, Bombyx mori. The studies in pharmacological activity found that sericin have some excellent properties; antimicrobial, antioxidant, antityrosinase, antitumor, uv resistant and moisture absorption. Material and method: in this study, we compared the pharmacological activity of sericin from different sources. One from UB1xUB5 extracted by hot water degumming at 100° c for 6 hours and other two sericin are available from commercial company (Chui Thai Silk and Kaewluang). The molecular weight of extracted sericin was evaluated by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The antioxidant activities of sericin (UB1xUB5, Chui Thai Silk and Kaewluang) were compared by 2,2-Diphenyl-1-picryl-hydrazil radical scavenging activity (DPPH Assay) and to determine antityrosinase activities using Dopachrome method. Results: The results showed that %yield of extracted sericin from UB1xUB5 was 12.60% w/w, molecular weight of UB1xUB5, Chui Thai Silk and Kaewluang were ranged between 30-250 kDa. For antioxidant activity was found that UB1xUB5 showed the highest %scavenging activity (70.00%), whereas Chui Thai Silk and Kaewluang were 16.67% and 23.33%, respectively. The antityrosinase activities of UB1xUB5, Chui Thai Silk and Kaewluang were 33.33%, 50.00% and 50.00%, respectively. Conclusion: The biological activities of sericin powder, including antioxidant activity and antityrosinase activity, which functions are important for potential applications of sericin to dietary supplements, food, and cosmetics industries.
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