The effect of vitamin E on oxidative stress and antioxidant capacity in blood and synovial fluid of patients with knee osteoarthritis
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Abstract
Introduction: Knee osteoarthritis (KOA), one of the most common degenerative joint diseases, is a major cause of morbidity in the elderly. Increasing evidences in both experimental and clinical researches suggest that oxidative stress plays an important role in the pathogenesis of osteoarthritis. Since vitamin E is one of the major dietary antioxidants, the objective of this study was to evaluate the effect of vitamin E supplementation on oxidative stress and antioxidant capacity in blood and synovial fluid from patients with knee osteoarthritis. Material and method: We measured lipid peroxidation products in 25 KOA patients by thiobarbituric acid reactive substances (TBARs), nitrite by Griess assay and total antioxidant capacity (TAC) by trolox equivalent antioxidant capacity (TEAC) assay and ferric reducing antioxidant power (FRAP) assay before and after 2 month supplementation with vitamin E 400 IU/day in plasma and synovial fluid. Results: After 2 months of supplementation, TBARs levels in plasma and synovial fluid were decreased significantly from 1.427 ± 0.075 µM to 0.999 ± 0.110 µM (P = 0.001) and 1.462 ± 0.123 µM to 1.124 ± 0.084 µM (P = 0.029), respectively. Although nitrite levels in plasma and synovial fluid were decreased, the differences were not statistically significant. Plasma and synovial fluid TEAC and FRAP levels were significantly increased after supplementation with vitamin E. Conclusion: Our findings showed that 2 month supplementation of vitamin E 400 IU/day provide positive outcome of plasma and synovial fluid in antioxidant status by decreasing TBARs levels and increasing TEAC and FRAP levels in patients with knee osteoarthritis. This results support a beneficial effect of vitamin E in the degenerative process of osteoarthritis. However, management should be under the care of knowledgeable physicians and dietitians.
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