Antioxidant and Melanogenesis stimulating activities of Some Thai Traditional Medicinal Plant Extracts for Grey Hair Treatment
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Abstract
Introduction : The objective of this study was to investigate antioxidant and melanogenesis stimulating activity of Thai traditional medicinal plant extracts included aqueous, ethyl acetate, methanol and hexane extracts of Tiliacora triandra, Centella asiatica, Clitoriaternatea, Morus alba and Pueraria mirifica. Material and Method : These extracts were determined for antioxidant activity by DPPH assay, ABTS assay, FRAP assay and also total phenolic compounds and determined for free radical protective activity of 2 mM hydrogen peroxide in mouse melanoma cells (B16F10) by colorimetric MTT assay. The melanogenesis stimulating activities were studied by the stimulation of tyrosinase enzyme activity by tyrosinase activity assay and melanocyte proliferation of these extracts at concentration of 0.75-800 pg/ml was evaluated using mouse melanoma cells (B16F10) by MTT assay. Results : For antioxidant activity. The aqueous extract of T. triandra exhibited strong antioxidant activity with the EC50 of 72.12 ± 20.18 pg/ml, TEAC of 0.77 ± 0.03 and VEAC of 2.00 ± 0.03, respectively. While the methanolic extract of T. triandra illustrated strong antioxidant activity with the FRAP value of 546.99 ± 0.01 ทาM and possessed the highest quantity of total phenolic compounds with GAE of 82.75. These extracts were also determined for free radical protective activity of 2 mM hydrogen peroxide. The results showed that aqueous extracts of M. alba, c. asiatica, T. triandra and c. ternatea had strong activities with % of cell viability of 88.29, 84.41, 83.14 and 76.25%, respectively.The stimulating activity of tyrosinase enzyme. The ethyl acetate extract of T. triandra had the strongest stimulating activity with % stimulation of 94.34. The stimulating activity of melanocyte proliferation of these extracts showed that methanol extract of c. ternatea and aqueous extract of T. triandra had strong stimulating activities with proliferation index (P.l.) of 1.7 and 1.6, respectively. Conclusion : The effect of five Thai medicinal plant extracts had melanogenesis stimulation and able to improved for anti-gray hair cosmetic in the future.
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