Anti-bacterial properties of propolis, royal jelly and Andrographis paniculata (Burm.f.) Nees against bacteria causing gastic ulcer, Helicobacter pylori
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Abstract
Introduction : Helicobacter pylori bacteria are a major cause of chronic gastritis, gastric ulcer and gastric cancer. These bacteria can be found throughout the world especially in developing countries. The current status shows that more than 50 percent of population in the world infected with these bacteria and almost all patients have symptom of chronic gastritis and gastric ulcer. Transmission of H. pylori likely occurs by consuming of contaminated food. Effective medications shows that patients need to get more than one type of antibiotics because higher chance of resistant bacteria against antibiotics occurs, thus treatment is not successful. Therefore, the use of natural extracts and medicinal plant is an alternative way that can replace the use of antibiotics and chemicals for prevention and treatment of gastritis disease caused by H. pylori infection. This study aims to investigate the inhibitory effect of propolis, royal jelly and Andrographis paniculata against H.pylori. Materials and Method : The inhibitory effects of propolis extract, royal jelly and A. paniculata extracts against the bacteria H. pylori clinical isolate strain H 54 and standard strain DMST 20165 were determined using agar disc diffusion method. Minimum inhibitory concentration (MIC) was determined by disc diffusion method and minimum bactericidal concentration (MBC) was determined by broth dilution method. Results : Propolis, royal jelly, and aqueous and ethanolic extracts of A. paniculata exhibited anti-/-/, pylori activity using disc diffusion method. The inhibition zone were within the range of 8-25 mm. Minimum inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC) were within the range of 7.8-500 mg/ml and 3.9500 mg/ml, respectively. Combinations of propolis extract, royal jelly and A. paniculata extracts were determined for anti-/-/. pylori activity. It was found that combination containing royal jelly inhibited H. pylori better than other combination without royal jelly. Conclusion : Propolis, royal jelly, and A. paniculata extracts demonstrated antibacterial activity against H. pylori strains DMST 20165 and H 54. Royal jelly showed the highest activity and H. pylori strain H 54 was inhibited higher than strain DMST 20165. Moreover, combination of substances containing royal jelly demonstrated higher anti-/-/, pylori activity when compared to other combination. H. pylori strains H 54 was also inhibited more than H. pylori strains DMST 20165 after treatment with combination of propolis, royal jelly, and extracts of A. paniculata.
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