Detection contaminate of Aflatoxin M1 in raw bulk milk and commercial milk within North-Eastern part of Thailand
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Abstract
Introduction: Aflatoxin M1 (AFM1) is also a hepatotoxic and carcinogenic in (IARC 2002) . Exposure to AFM1 throuhg milk and milk product is a serious problem for public health. The present study was detected Aflatoxin M1 in raw bulk milk and commercial milk within North-Eastern part of Thailand. Material and method: Randomized raw milk 92 samples from 46 farms and commercial milk 23 samples are produced in North-Eastern region of Thailand in November and December 2011. Concentration Aflatoxin M.| in milk was validated with Competitive Eenzyme Linked Immunosorbant Assay (ELISA) method and use ANOVA for statistical testing. Results: The 111 samples found contamination of AFM1 (96.26%) from all 115 samples. The average of concentration is 0.285 ±0.262 ng/ml , in raw milk found contaminate all of samples (92 samples) and the concentration between 0.012-0.973 ng/ml (average 0.331 ± 0.264 ng/ml), in the commercial milk found contaminated 19 samples(82.60%) from 23 samples of all have a concentration between 0.04-0.680 ng/ml (average 0.099 ± 0.154 ng/ml). Conclusion : In the present study have 111 samples were positive result from detection aflatoxin M.| in milk . 77.39% of sample have higher Aflatoxin M1 concentration level than the regulatory limit for AFM1 in European Community (< 0.05 ng/ml) and 23.48% of sample have higher level than limit of USA (< 0.5 pg/L). Even the concentration level of Aflatoxin M.| is low but when the consumers have recieved milk was Aflatoxin M.| contaminate always that can effect toxication to health. So the regulatory limit for Aflatoxin M.| are needed to regulate and improve quality of raw milk and milk product in Thailand include provided knowledge for owner for decrease the contamination of Aflatoxin M.| in milk
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References
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