Curcuma comosa Roxb Extract loaded Nanostructured Lipid Carriers for Enhancement of Skin Permeation

Main Article Content

Damrongsak Jinarat
Bancha Yingngam
Wandee Rungseevijitprapa


Introduction: Curcuma comosa composed of phytoestrogenic compounds that have been reported to have several bioactivities. However, the scientific support the use of Curcuma comosa in dermal applications are still limited. The purposes of this research were to investigate the potential of skin bioactivities of C. comosa and develop nanostructure lipid carrier system for transcutaneous delivery. Methods: Curcuma comosa was extracted in n-hexane. Skin bioactivities of the extract were investigated include antioxidation, tyrosinase inhibitor and in vitro collagenase inhibition. Nanostructure lipid carrier systems were developed. Effect of solid lipid, oil as well as chemical skin permeation enhancers on particle characterization, % encapsulation efficiency, thermal property of lipid, in vitro skin permeation ability by Franz diffusion cells and stability of the formulations were evaluated. Results: The obtained C. comosa extract from hexane have concentrated semisolid characteristic rich in phytoestrogen. Skin bioactivities compared with the controls were mild activities. The developed NLC systems had the particle size in the range of 120 – 170 nm, PDI 0.25 - 0.29, approximate zetapotential was -20 mV, higher %EE than 90, good physical stable when kept at 25 and 40 ˚C for 90 days. After 24 hours, chemical enhancers facilitated 3.2 to 5.3% of the active DA2 through the stratum corneum. The lipophilic property of C. comosa extract limited DA2 skin permeation and the active was mostly accumulated within the epidermis skin layer. Conclusion: Curcuma comosa extract loaded NLC has a potential to be further developed as transdermal delivery of phytoestrogenic compound for local action of hormone replacement therapy with low systemic side effect.


Download data is not yet available.

Article Details

Pharmaceutical Sciences


Adithya ES, Lakshmi MS, Christabel PH, Sasikumar JM. In vitro antioxidant, anti-lipid peroxidation activities and HPLC analysis of methanol extracts from bark and stem of Mahonia leschenaultia takeda. Asian J Plant Sci Res 2013; 3(2): 116-126.

Almeida ED, Costa AA, Serafini MR, Rossetti FC, et al. Preparation and characterization of chloroaluminum

Chun KS, Sohn Y, Kim HS, Kim OH, et al. Anti-tumor promoting potential of naturally occurring diarylheptanoids structurally related to curcumin. Mutat Res 1999; 428(1-2): 49-57.

Frasor J, Barnett DH, Danes JM, Hess R, Parlow AF, Katzenellenbogen BS. Response-specific and ligand dose-dependent modulation of estrogen receptor (ER) alpha activity by ERbeta in the uterus. Endocrinology 2003; 144(7): 3159–3166.

Golden GM, McKie JE, Potts RO. Role of stratum corneum lipid fluidity in transdermal drug flux. J Pharm Sci 1987; 76(1), 25–28.

Gunasekaran T, Haile T, Nigusse T, Dhanaraju MD. Nanotechnology: an effective tool for enhancing bioavailability and bioactivity of phytomedicine. Asian Pac J Trop Biomed 2014; 4(Suppl 1): S1–S7.

Gunullu U, Uner M, Yener G, et al. Formulation and characterization of solid lipid nanoparticles, nanostructured lipid carriers and nanoemulsion of lornoxicam for transdermal delivery. Acta Pharm 2015, 65: 1-13.

Ichikawa K, Sasada R, Chiba K, Gotoh H. Effect of side chain functional groups on the DPPH radical scavenging activity of bisabolane-type phenols. Antioxidants 2019; 8(65).

Jantaratnotai N, Utaisincharoen P, Piyachaturawat P, et al. Inhibitory effect of Curcuma comosa on NO production and cytokine expression in LPS-activated microglia. Life Sci 2006, 78: 571-577.

Jeon SH, Kim KH, Koh JU, Kong KH. Inhibitory effects on L-Dopa oxidation of tyrosinase by skin-whitening agents, Bull Korean Chem Soc 2005; 26(7): 1135-1137.

Jinarat D, Yingngam B, Suksamrarn A, Rungseevijitprapa W. Development and characterization of Curcuma comosa extract loaded nanostructured lipid carriers. IJPS 2017;13(Supplement):163-172.

Katakam P, Kumari AP, Hwisa N, Chandu BR. Development of stability indicating media for in-vitro dissolution testing of didanosine in pharmaceutical dosage forms. Res Sci Tech. 2011; 3: 41-45.

Kopper NW, Gudeman J, Thompson DJ. Transdermal hormone therapy in postmenopausal women: A review of metabolic effects and drug delivery technologies. Drug Des Devel Ther 2009; 2: 193–202.

Küchler S, Herrmann W, Panek-Minkin G, Blaschke T, et al. SLN for topical application in skin diseases-characterization of drug-carrier and carrier-target interactions. Int J Pharm. 2010; 390(2):225-33.

Kundu S, Wang Y, Xia W, Mathur M. Thermal stability and reducibility of oxygen-containing functional groups on multiwalled carbon nanotube surfaces: A quantitative high-resolution XPS and TPD/TPR study. J. Phys. Chem. 2008;1129(43):16869-16878.

Markownikoff W. Ueber die Abhängigkeit der verschiedenen Vertretbarkeit des Radicalwasserstoffs in den isomeren Buttersäuren. Annalen der Pharmacie. 1870; 153 (1): 228–59.

N’Da D. Prodrug Strategies for enhancing the percutaneous absorption of drugs. Molecules 2014; 19(12): 20780–20807.

phthalocyanine-loaded solid lipid nanoparticles by thermal analysis and powder X-ray diffraction techniques. J Therm Anal Calorim. 2012; 108: 191–196.

Piyachaturawat P, Ercharuporn S and Suksamrarn A. Estrogenic activity of Curcuma comosa extract in rat, Asia Pac J Pharm 1995;10:121-126.

Prasanthi D, Lakshmi P K. Terpenes: Effect of lipophilicity in enhancing transdermal delivery of alfuzosin hydrochloride. J Adv Pharm Technol Res 2012;3(4):216–223.

Pritchard KI. Breast cancer prevention with selective estrogen receptor modulators: a perspective. Ann NY Ace sci 2001;949:89-98.

Rangsimawong W, Tansathien K, Nuntharatanapon N, Jaewjira S, Opanasopit P. Development of Pueraria mirifica extract-loaded lipid nanoparticles for hair spray. Thai Bull Pharm Sci 2017; 12(2):13-20.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, & Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999; 26(9–10):1231–1237.

Sakdiset P, Kitao Y, Todo H, Sugibayashi K. High-throughput screening of potential skin penetration-enhancers using stratum corneum lipid liposomes: Preliminary evaluation for different concentrations of ethanol. J Pharm 2017;7409420.

Sator PG. Skin treatments and dermatological procedures to promote youthful skin. Clin Interv Aging 2006; 1(1): 51-6.

Sodsai A, Piyachaturawat P, Sophasan S, Suksamrarn A, Vongsakul M. Suppression by Curcuma comosa Roxb. of pro-inflammatory cytokine secretion in phorbol-12-myristate-13-acetate stimulated human mononuclear cells. Int Immunopharmacol 2007; 7: 524–531.

Souto EB, Müller RH. Cosmetic features and applications of lipid nanoparticles (SLN, NLC). Int J Cosmet Sci. 2008; 30(3): 157-65.

Su J, Sripanidkulchai K., Hu Y, Chaiittianan R, Sripanidkulchai, B. Increased In Situ Intestinal Absorption of Phytoestrogenic Diarylheptanoids from Curcuma comosa in Nanoemulsions. AAPS PharmSciTech 2013; 14(3): 1055–1062.

Su J, Sripanidkulchai K., Suksamrarn A., Hu Y, Piyachuturawat P,Sripanidkulchai B. Pharmacokinetics and organ distribution of diarylheptanoid phytoestrogens from Curcuma comosa in rats. J Nat Med 2012; 66(3): 468-475.

Suksamrarn A, Ponglikitmongkol M, Wongkrajang K, Chindaduang A, et al. Diarylheptanoids, new phytoestrogens from the rhizomes of Curcuma comosa: Isolation, chemical modification and estrogenic activity evaluation. Bioorg Med Chem 2008; 16(14): 6891-902.

Tantikanlayaporn D, Wichit P, Weerachayaphorn J, Chairoungdua A, et al. Bone Sparing Effect of a Novel Phytoestrogen Diarylheptanoid from Curcuma comosa Roxb. in Ovariectomized Rats. PLoS ONE 2013; 8(11): e78739

Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byne DH. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts, J Food Comp Anal 2006; 19(6-7): 669-675.

Tuntiyasawasdikul S, Limpongsa E, Jaipakdee N, Sripanidkulchai B Effects of Vehicles and Enhancers on the Skin Permeation of Phytoestrogenic Diarylheptanoids from Curcuma comosa. AAPS PharmSciTech 2017; 18(3): 895-903.

Tuntiyasawasdikul S, Limpongsa E, Jaipakdee N, Sripanidkulchai B. Development and evaluation of topical films containing phytoestrogenic diaryheptanoids from Curcuma comosa extract. Drug Dev Ind Pharm 2018; 44(8): 1385–1394.

Watkins R, Wu L, Zhang C, Davis R, Xu B. Natural product-based nanomedicine: recent advances and issues. Int J Nanomed 2015: 6055-6074.

Winuthayanon W, Piyachaturawat P, Suksamrarn A, Ponglikitmongkol M, et al. Diarylheptanoid phytoestrogens isolated from medicinal plant Curcuma comosa: Biologic actions in vitro and in vivo indicate estrogen receptor-dependent mechanisms, Environ Health Perspec 2009; 11(7): 1155-1161.

Wissing SA, Müller RH. Cosmetic applications for solid lipid nanoparticles (SLN). Int J Pharm. 2003; 254: 65–68.

Yameen B, Choi WI, Vilos C, Swami A, et al. Insight into nanoparticle cellular uptake and intracellular targeting. J Control Release 2014; 190: 485-499.

Yingngam B, Brantner AH, Jinarat D, Kaewamatawong R, et al. Determination of the marker diarylheptanoid phytoestrogens in Curcuma comosa rhizomes and selected herbal medicinal products by HPLC-DAD. Chem Pharm Bull 2018; 66(1): 65-70.

Yingngam B, Rungseevijitprapa W. Molecular and clinical role of phytoestrogens as anti-skin-ageing agents: A critical overview. Phytopharmacology 2012; 3(2): 227-244.