Study of The Extraction Method for Chloramphenicol Residues in Chicken Samples by Solid-phase Extraction and Solvent Extraction

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Supawadee Daodee
Jinda Wangboonskul
Tave Promdee
Wimonsri Telvong
Mongkol Kantapanichakan


Chloramphenicol is a broad-spectrum antibiotic which have a strictly regulation for importation and is not allowed to feed the animals for consumption in Thailand because of the toxicity in humans. The purpose of this study was to determine the appropriate extraction method to detect the chloramphenical residue in chicken samples. Solid-phase and liquid extraction were used in this study. To detect chloramphenicol, high performance liquid chromatographic method combined with ultraviolet-visible detector was used. From the result of this study, it could be concluded that the combination of solid-phase extraction and liquid extraction was appropriate and gave a good result for detecting the chloramphenicol residue in chicken. The method showed significantly reduce the interference from endogeneous substances. All 24 chicken samples collected from the markets in Muang district, Khon Kaen province, were not found chloramphenicol residue.


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