Comparative Mucin Production in Biliary and Intestinal Epithelium of Opisthorchiasis-Susceptible and Non-Susceptible Animal Models
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Abstract
Objectives: This study aimed to investigate the mucin production change within biliary and intestinal epithelia following Opisthorchis viverrini infection in susceptible and non-susceptible animal models.
Materials and Methods: We examined archived paraffin blocks from a time-series study involving two animal models of opisthorchiasis. Hamsters and Balb/c-R/J mice were categorized as susceptible and non-susceptible models, respectively. Histopathological and histochemical (AB-PAS staining) techniques were used to assess mucin production, specifically the mucin index, in both biliary and intestinal epithelia. Statistical analysis was performed to compare differences between epithelium types in both animal models and between non-infection (NI) and O. viverrini-infected (OV) groups.
Results: Goblet cell metaplasia of biliary epithelium was detected in the OV group of both animal models. The response was early in the mice model and late in the hamster model. Histochemical examination revealed mixed-type mucin in hamster bile duct and villous of duodenum, with acid-type mucin in duodenal submucosal glands and neutral-type among zymogen granules. Mice predominantly displayed acid-type mucin in the bile duct and mixed-type mucin in the intestine. The intestinal epithelium consistently exhibits a higher mucin index than the hamster's bile duct. In contrast, no statistical difference exists between mice's biliary and intestinal epithelia after day 2 post-infection.
Conclusions: Goblet cell metaplasia was observed as a response in the biliary epithelium, producing mucin in response to OV infection of both susceptible and non-susceptible models. This response was less pronounced in the intestine.
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