Antimicrobial residues in chicken meat and livers sold in six northeastern Thai provinces by screening test, CM-Test and RR-test®
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Abstract
Objective: The aim of this study was to survey antibiotic residues in commercial broiler farms’ chicken meat sold in the markets of 6 northeastern Thai provinces and in backyard farms’ chicken meat and liver sold in Mueang district, Khon Kaen.
Materials and Methods: From August to September 2018, a total of 600 chicken meat samples of commercial farms were collected from the markets in 6 provinces (100 samples per province): Udon Thani, Nakhon Ratchasima, Ubon Ratchathani, Sisaket, Mukdahan, and Khon Kaen. An additional 50 samples (chicken meat = 25, livers = 25) of backyard farms were collected from the markets in Khon Kaen municipal during December 2018. The microbial inhibition assays were used for antimicrobial residual detection, CM-Test and RR-test®.
Results: No antibiotic residues were found in all 600 chicken meat samples from standard commercial farms, while chicken meat and liver from backyard farms yielded positive at 28% (14/50), which was found in chicken liver (44%) rather than chicken meat (12%). This accounts for 4% of Tetracyclines and 24% of Macrolides-Aminoglycosides-Sulfonamides drug classes.
Conclusion: Antimicrobial residues in chicken liver from backyard farms sold in Mueang district, Khon Kaen revealed at a higher rate than in chicken meat. Consuming chicken liver poses a higher risk of antibiotic residues than chicken meat. As a result, controlling antimicrobial usage in small farms is critical to avoiding drug residues.
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References
Chalermchaikit T, Poonsook K, Dangprom K, Lertworapreecha M, Jotisakulratana K, 2002. Efficiency of antimicrobial residue screening test kit for meat “CM-Test”. Agricultural Sci J 33:6 (Suppl.), 376-379.
Chalermchaikit T, Lertworapreecha M, Poonsook K, Lertworapreecha N, Srisanga S, Jotisakulratana K, 2003. “CM-Test” (Clean Meat-Test): The new antimicrobial residue screening test kit in meat. The 11th International Symposium of the World Association of Veterinary Laboratory Diagnosticians and OIE Seminar on Biotechnology, November 9-13.
Department of Medical Sciences, Ministry of Public Health, 2018. Test kit for antimicrobial residual detection in milk and milk products. [Cited 2022 July 28]. Available from: http://testkit.dmsc.moph.go.th/page-view/110
Department of International Trade, Ministry of Commerce, Thailand, 2021. Broiler situation. [Cited 2022 July 28]. Available from: http://agri.dit.go.th/index.php/department_doc/6/
Ferdous MRA, Ahmed MR, Khan SH, Mukta MA, Anika TT, Hossain MT, Islam MZ, Rafiq K, 2020. Effect of discriminate and indiscriminate use of oxytetracycline on residual status in broiler soft tissues. Vet World 13, 61–67. https://doi.org/10.14202/vetworld.2020.61-67
Hussein MA, Ahmed MM, Morshedy AM, 2016. Effect of cooking methods on some antibiotic residues in chicken meat. Jpn J Vet Res 64, S225–S231.
Lekagul A, Tangcharoensathien V, Mills A, Rushton J, Yeung S, 2020. How antibiotics are used in pig farming: A mixed-methods study of pig farmers, feed mills and veterinarians in Thailand. BMJ Glob Heal 5, 1918. https://doi.org/10.1136/bmjgh-2019-001918
Muaz K, Riaz M, Akhtar S, Park S, Ismail A, 2018. Antibiotic residues in chicken meat: Global prevalence, threats, and decontamination strategies: A review. J Food Prot 81, 619–627. https://doi.org/10.4315/0362-028X.JFP-17-086
Mund MD, Khan UH, Tahir U, Mustafa BE, Fayyaz A, 2017. Antimicrobial drug residues in poultry products and implications on public health: A review. Int J Food Prop 20, 1433–1446. https://doi.org/10.1080/10942912.2016.1212874
Nakrintr C, Chatsuparp A, 2002. Detection of antibiotic residues in pork, beef, and chicken meat in Chaiyaphum province. Independent study. Chaiyaphum Provincial Livestock Office.
Open-Source Epidemiologic Statistics for Public Health, Version 3.01, 2013. [Cited 2018 June 20]. Available from: https://www.openepi.com/Menu/OE_Menu.htm
Sarker YA, Hasan MM, Paul TK, Rashid SZ, Alam MN, Sikder MH, 2018. Screening of antibiotic residues in chicken meat in Bangladesh by thin layer chromatography. J Adv Vet Anim Res 5, 140–145. https://doi.org/10.5455/javar.2018.e257
Shahbazi Y, Ahmadi F, Karami N, 2015. Screening, determination, and confirmation of tetracycline residues in chicken tissues using four-plate test, ELISA and HPLC-UV methods: comparison between correlation results. Food Agric Immunol 26, 821–834. https://doi.org/10.1080/09540105.2015.1036357
Sichilongo KF, Muckoya VA, Nindi MM, 2015. A Rapid and Sensitive LC-MS/MS Method for the Determination of Multi-class Residues of Antibiotics in Chicken Liver. South African J Chem. 68. https://doi.org/10.17159/0379-4350/2015/v68a1
Smart Buying magazine, 2018. Monitoring of goods and services for protecting of consumers’ health. [Cited 2022 July 28]. Available from https://embed.voicetv.co.th/read/SyRlJ0A7Q
Thi N, Thi N, Van B, Cuong N, Van TT, Duong Q, 2018. Antimicrobial residues and resistance against critically important antimicrobials in non-typhoidal Salmonella from meat sold at wet markets and supermarkets in Vietnam. Int J Food Microbiol 266, 301–309. https://doi.org/10.1016/j.ijfoodmicro.2017.12.015
Xu L, Wang W, Xu W, 2022. Effects of tetracycline antibiotics in chicken manure on soil microbes and antibiotic resistance genes (ARGs). Environ Geochem Health 44. https://doi.org/10.1007/s10653-021-01004-y