The study of chemical composition, physical characteristic and cytotoxicity of Thai bentonite The study of chemical composition, physical characteristic and cytotoxicity of Thai bentonite
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Abstract
Objectives: The aims of this study were to investigate the structurally chemical and physical properties, and to evaluate primary cytotoxicity of Thai bentonite on Vero cell line.
Materials and Methods: The structure of Thai bentonite was investigated by measuring pH, cation exchange capacity (CEC), specific surface area, swelling index, crystal structure, functional group, surface morphology and elemental composition, while the cytotoxicity of Thai bentonite was conducted by MTT assay on Vero cell line.
Results: The results revealed that Thai bentonite had chemical and physical properties as reported of another bentonite, and it had high adsorption property because of high CEC, specific surface area and swelling index. In addition, it had no contamination of harmful element. Thai bentonite showed the low cytotoxicity level on Vero cell line as presenting the half-maximal inhibitory concentration at 66, 20 and 15 mg/L after exposure at 24, 48 and 72 h, respectively.
Conclusion: All results indicated that Thai bentonite had the great adsorption property with safe application in organism, and it also contained supplementarily necessary elements for animals.
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