The Application of Near-infrared spectroscopy technique for measuring plasma Beta-hydroxybutyrate and non-esterified fatty acid concentration and subclinical ketosis screening test in dairy cow

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theerakul Nilnont
Rittichai pilachai
Pranpreya Todtong
Saranya Workhwa

Abstract

Introduction: Ketosis is one of the metabolic diseases that causes economic loses in dairy industry. The evaluation of BHB and NEFA is a specific test for subclinical ketosis which shows the higher blood level of beta-hydroxybutyrate (BHB) and non-esterified fatty acid (NEFA). There have been applied the Near-infrared spectroscopy (NIRS) technique for detecting subclinical ketosis, however the studies of this technique are needed for supporting the accuracy of the analysis and screening tests.


Objectives: The aims of this study were to apply the NIRS technique to measure plasma BHB and NEFA of dairy cow by generating the appropriate equation to predict the relationship between the laboratory BHB and NEFA results and the NIRS results, and to investigate the prevalence of subclinical ketosis in dairy raising area, Udon Thani province.


Materials and methods: blood and plasma samples of 102 post-partum dairy cows (5-60 DIM) were collected. The plasma samples were separated and divided into 2 parts, for laboratory BHB and NEFA analysis and NIRS analysis by using FT-NIR and DLP-NIR. The appropriate equation was generated to predict the BHB and NEFA level.    


Results: The prevalence of subclinical ketosis in pots-partum dairy cows was 5.88% (6/102). The statistical analysis to generate the equation to predict plasma BHB and NEFA level, by using FT-NIR และ DLP-NIR, demonstrated the R value were 0.98, 0.90 and 0.98, 0.84, respectively.


Conclusions: The results of this study showed the moderate relationship between the laboratory and the NIRS BHB and NEFA level and was able to apply in the research. The further studies and data collections is necessary for improve the accuracy of blood chemistry analysis by using NIRS technique.

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