Prevalence and antimicrobial susceptibility on bacterial pathogen of clinical and subclinical mastitis in lactating cows In Udon Thani Province

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Pranpreya Todtong
theerakul Nilnont
Rittichai Pilachai

Abstract

Introduction: Mastitis is a major problem persisted with dairy farmers for a long time, and results to economic impact of dairy farming around the world and Thailand. The incidence of subclinical mastitis was high and antimicrobials treatment were low effective to mastitis in this area.


Objectives: To study the prevalence and antimicrobial susceptibility on bacterial pathogen of clinical and subclinical mastitis in lactating cows in Udon Thani province.


Materials and Methods: Milk samples were collected from lactating cows from 50 farms with mastitis during March-July 2016, 318 samples, divided into 2 groups: 1) 57 clinical mastitis and 2) 261 subclinical mastitis. The samples were cultured and tested for susceptibility of pathogens to antimicrobials by the Agar Disk Diffusion method.


Results: The prevalence of clinical mastitis was 17.92% and subclinical mastitis was 82.08%. Staphylococcus spp. were the most common pathogens causing both clinical (63.16%) and subclinical mastitis (59.39%). Streptococcus spp. (17.54% and 14.94%), and Bacillus spp. (15.79% and 16.09%). Enterobacteriaceae found only in the group with subclinical mastitis (3.45%). The mean of antimicrobials susceptibility to bacterial pathogen of clinical and subclinical mastitis were ceftiofur (87.50%), kanamycin (87.18%), and gentamycin (86.60%).


Conclusions: The lactating cows at Si That district Udon Thani province were found the prevalence of subclinical higher than the clinical mastitis. The main pathogenic bacteria were Staphylococcus spp., Streptococcus spp. and Bacillus spp. The antimicrobial agents with mean susceptibility to bacteria cause of clinical and subclinical mastitis were susceptible to ceftiofur, antimicrobial agent in this group is the highest susceptibility. Therefore, it was a good treatment option in this area.

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References

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