Prevalence and antimicrobial susceptibility on bacterial pathogen of clinical and subclinical mastitis in lactating cows In Udon Thani Province
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Abstract
Introduction: Mastitis is a major problem persisted with dairy farmers for a long time, and results to economic impact of dairy farming around the world and Thailand. The incidence of subclinical mastitis was high and antimicrobials treatment were low effective to mastitis in this area.
Objectives: To study the prevalence and antimicrobial susceptibility on bacterial pathogen of clinical and subclinical mastitis in lactating cows in Udon Thani province.
Materials and Methods: Milk samples were collected from lactating cows from 50 farms with mastitis during March-July 2016, 318 samples, divided into 2 groups: 1) 57 clinical mastitis and 2) 261 subclinical mastitis. The samples were cultured and tested for susceptibility of pathogens to antimicrobials by the Agar Disk Diffusion method.
Results: The prevalence of clinical mastitis was 17.92% and subclinical mastitis was 82.08%. Staphylococcus spp. were the most common pathogens causing both clinical (63.16%) and subclinical mastitis (59.39%). Streptococcus spp. (17.54% and 14.94%), and Bacillus spp. (15.79% and 16.09%). Enterobacteriaceae found only in the group with subclinical mastitis (3.45%). The mean of antimicrobials susceptibility to bacterial pathogen of clinical and subclinical mastitis were ceftiofur (87.50%), kanamycin (87.18%), and gentamycin (86.60%).
Conclusions: The lactating cows at Si That district Udon Thani province were found the prevalence of subclinical higher than the clinical mastitis. The main pathogenic bacteria were Staphylococcus spp., Streptococcus spp. and Bacillus spp. The antimicrobial agents with mean susceptibility to bacteria cause of clinical and subclinical mastitis were susceptible to ceftiofur, antimicrobial agent in this group is the highest susceptibility. Therefore, it was a good treatment option in this area.
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Aiumlamai S., Chanlun A., Sakolapong V., van Oosteveen F., Jarassaeng C., Krajangpo C., Pattanawong J, 2000. Prevalence of organisms causing subclinical mastitis in Amphur Muang, Khon Kaen. Proceedings of the 26th Annual conference the Thai Veterinary Medical Association Udder the Royal Patronage, Bangkok, Thailand, 15-17 November. p. 53-63.
Al-Mutairi M.F, 2011. The incidence of Enterobacteriace causimg food poisoning in some meat product. J Food Sci Technol 3(2), 116-121.
Ard-ong O, Parinya P, 2011. An evaluation of bulk milk somatic cell count (BMSCC) and its impact on milk production in dairy farm of the upper Northeastern part of Thailand in the fiscal year 2009. Thai-NIAH e-Journal 5 (3), 87-97.
Bartlett PC, Miller GY, Lance SE, Heider LE, 1992. Clinical mastitis and intramammary infections on Ohio dairy farms. Prev Vet Med 12, 59-71.
Bradley AJ, GreenMJ, 2005. Use and interpretation of somatic cell count data in dairy cows. In Pract 27, 310-315.
Busato A, Trachsel P, Schallibaum M, Blum JW, 2000. Udder health and risk factors for subclinical mastitis in organic dairy farms in Switzerland. Prev Vet Med 44, 205-220.
Dodd FH, 1983. Mastitis-Progress on control. J Dairy Sci 66, 1773-1781.
Eberhart JL, Luoma DL, 1996. Truncocolumella citrina + Pseudotsuga menziesii. In: Goodman, D.M., Durall, D.M., Trofymow, J.A., and Berch, S.M. (eds.). A manual of concise descriptions of North American ectomycorrhizae. Mycologue Publications, copublished by B.C. Ministry of Forests, Canadian Forest Service, Victoria B.C. Pp. CDE9, 1-4.
Jay, J.M. (Ed.), 1996. Modern Food Microbiology. 5th ed. New York: Chapman & Hall.
Jayarao BM, Gillespie BE, Lewis MJ, Dowlen HH, Oliver SP, 1999. Epidemiology of Streptococcus uberis intramammary infections in a dairy herd. J Vet Med 46, 433-442.
Kirk JH, Wright JC, Berry WL, Reynolds JP, Maas JP, Ahmadi A, 1996. Relationships of milk culture status at calving with somatic cell counts and milk production of dairy heifers during early lactation on a Californian dairy. Prev Vet Med 28, 187-198.
Krukowski H, Tietze M, Majewski T, Rozanski P, 2001. Survey of yeast mastitis in dairy herds of small type farms in the Lublin region, Poland. Mycopathologia 150, 5-7.
Lescourret F, Coulon JB, Faye B, 1995. Predictive model of mastitis occurrence in the dairy cow. J Dairy Sci 78, 2167-77.
MacKellar, Q.A, 1991. Intramammary treatment of mastitis in cows. In Pract 13, 244–249.
National Committee for Clinical Laboratory Standards, 2000. Methods for dilution antimicrobial susceptibility test for bacteria that grow aerobically; Approved Standard- Fifth Edition. NCCLS document M7- A5 (ISBN 1-56238-394-9). NCCLS,940 West Vally Road, Suite 1400, Wayne, Pennsylvania 19087- 1898, USA.
National Committee for Clinical Laboratory Standards,1997. Methods for dilution antimicro-bial susceptibility tests for bacteria that grow aerobically, Approved standard M7-A4. National Committee for Clinical Labora-tory Standards, Wayne, Pa.
National Mastitis Council (NMC),1999. Laboratory handbook on bovine mastitis. 2nded. Verona: National Mastitis Council, Inc.
Natthamon T, 2013. Bulk tank somatic cell count and bacteria standard plate count from bovine milk in northeastern region of Thailand. Agricultural Sci J 44(1), 391-394.
Natzke RP. Elements of mastitis control. J Dairy Sci 64, 1431.
Oliver SP, 1988. Frequency of isolation of environmental mastitis-causing pathogens and incidence of new intramammary infection during the nonlactating period. Am J Vet Res 49, 1789-1793.
Philpot WN, Nickerson SC, 1991. Mastitis: Counter Attach. Babson Bross.Co. Illinois.USA; p 150.
Phuektes P, Mansel PD, Rodney SD, Hooper ND, Dick JS, Browning GF, 2001. Molecular epidemiology of Streptococcus uberis isolates from dairy cows with mastitis. J Clin Microbiol 39, 1460-1466.
Rittichai P, Sudawan C, Pranpreya K, 2014. Prevalence of subclinical mastitis and potential risk factors in lactating cows In Udon Thani Province. KKU Vet J 24(1), 43-56
Schrick FN, Hockett ME, Saxton AM, Lewis MJ, Dowlen HH, Oliver SP, 2001. Influence of subclinical mastitis during early lactation on reproductive parameters. J Dairy Sci 84, 1407-1412.
Schukken YH, Lam TJ, Nielen M, Hegeveen H, Barkema HW, Grommers FJ, 1995. Subclinical mastitis on dairy farms in the Netherlands: epidemiological developments. Tijdschrift v Diergen 120, 208-213.
Seegers H, Fourichon C, Beaudeaup F, 2003. Production effects related to mastitis and mastitis economics in dairy cattle herds. Vet Res 34, 475-491.
Sukolrat B, Julanee T, Khwanchai K, Witaya S, 2007. Anitimicrobial Resistance of Mastitis-Associated Bacteria in Lactating Dairy Cows in Chiang Mai Province. Chiang mai Vet J 5(2), 135-145.
Vasavada, P.C., Cousin, M.A, 1993. Dairy microbiology and safety. In Hui, Y.H. (Ed.) Dairy Science and Technology Handbook, Vol. 2: Product Manufacturing. (pp. 301-426). New York: VCH Publishers.