The selection of exopolysaccharides-producing lactic acid bacteria from the Thai traditional fermented food and animal gastrointestinal tracts
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Abstract
Objective: The objective of the present study was to select lactic acid bacteria (LAB) with probiotic properties and abil- ity to produce exopolysaccharides (EPS) from the animal gastrointestinal tracts and Thai traditional fermented foods. These LAB isolates aimed to use in animal production and fermented food industry.
Materials and Methods: The study samples were selected from five sources: the swine gut, the chicken gut, Nham- moo, Mum-wua, and Pla-som. LAB isolates were selected based on their properties in catalase production, acid and alkali tolerance, bile salt tolerance, antimicrobial activity, antibiotic susceptibility, and EPS production. The EPS produc- tion was tested by using phenol-sulphuric method.
Results: LAB isolates were 152 from the swine gut, 161 from the chicken gut, 160 from Nham-moo, 174 from Mum-wua and 158 from Pla-som. The catalase production test showed that 761 isolates were catalase-negative. Of these, 176 isolates survived at pH 2, 4 and 10 for 3 and 6 hours. These 176 isolates also resisted to amoxicillin-clavulanic acid, oxytetracycline, streptomycin, vancomycin and trimethoprim-sulfamethoxazole at 4.54%, 50.57%, 65.34%, 73.86% and 68.18%, respectively. Of 176 isolates, 78 isolates showed strong inhibition to E. coli ATCC 25922, Salmonella Typhi and Listeria monocytogenes at 5.12%, 5.12%, 8.97%, respectively and moderate inhibition to E. coli ATCC 25922, S. Typhi and Listeria monocytogenes at 60.25%, 60.25%, 76.92%, respectively. Four selected isolates F3-3, N45-1, C57 and M60-3 showed the highest EPS yield at 3.21, 2.54, 2.36 and 2.11 grams per liter, respectively.
Conclusion: SFour isolates F3-3, N45-1, C57 and M60-3 were selected based on their EPS production for further study in animal production and fermented food industry.
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