A Study of Antimicrobial Activity of Oxystelma esculentum R. Br. and Paederia linearis Hook. f. Extracts Against Bacteria and Yeasts
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Abstract
Objective To study antimicrobial activity of Oxystelma esculentum R. Br. and Paederia linearis Hook. f. extracts against bacteria and yeast, and determine their minimum inhibitory concentrations.
Materials and methods Oxystelma esculentum R. Br. and Paederia linearis Hook. f. were collected, dried and crushed into fine powdered. Then the powdered samples were extracted by 95% ethanol. After that, bacteria and yeast were isolated from animals and cultured on agar to test for the antimicrobial activity of Oxystelma esculentum R. Br. and Paederia linearis Hook. f. extracts against bacteria and yeasts by using broth microdilution method. Gentamicin and ketoconazole, Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 were also tested as controls.
Result Oxystelma esculentum R. Br. showed highest antimicrobial activity against S. aureus, followed by S. aureus ATCC 25923, Streptococcus agalactiae, Klebsiella spp., Staphylococcus hyicus, Aeromonas hydrophila, Staphylococcus intermedius, Pseudomonas aeruginosa, E. coli ATCC 25922 with the minimal inhibitory concentrations (MICs) of 0.38, 6.07±68.32, 6.07±68.32, 9.63±50.33, 12.14±67.51, 17.14±66.45, 17.14±66.45, 34.30±60.01 and 48.50±51.44 µg/ml respectively. From the result, Oxystelma esculentum R. Br., also exhibited the higher antifungal activity against Cryptococcus neoformans than Candida albicans with MICs of 6.06±12.25 and 34.29±17.15 µg/ml respectively. For Paederia linearis Hook. f., it exhibited the highest antimicrobial activity against S. aureus ATCC 25923 and S. aureus; followed by S. hyicus, S. intermedius, S. agalactiae, A. hydrophila, E. coli ATCC 25922, Klebsiella spp., P. aeruginosa with MICs of 0.88, 0.88, 1.05±0.45, 2.80±3.11, 8.90±15.84, 14.15, 28.31, 28.31, 28.31 µg/ml respectively; and Paederia linearis Hook. f. also exhibited higher antifungal activity against C. neoformans than C. albicans with minimal inhibitory concentrations of 22.46±24.52 and 35.66±53.59 µg/ml, respectively.
Conclusion Oxystelma esculentum R. Br. and Paederia linearis Hook. f. could inhibit the growth of many types of bacteria, including S. aureus, S. aureus ATCC 25923, S. agalactiae, Klebsiella spp, S. hyicus, A. hydrophila, S. intermedius, P. aeruginosa, E. coli ATCC 25922 with various MIC values and could also inhibit the growth of fungi such as C. albicans and C. neoformans; and the antimicrobial activity between Oxystelma esculentum R. Br. and Paederia linearis Hook. f. were not different statistically. (P> 0.05)
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References
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