Monitoring of Burkholderia pseudomallei in animals from slaughterhouses in Nakhon Pathom and Ratchaburi provinces
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Abstract
Objective - To study of Burkholderia pseudomallei from animals slaughtered in slaughterhouses, these data would benefit to stakeholders in biosafety managements.
Materials and methods - A total of 400 samples were collected by purposive sampling from organs and tissues containing gross lesion, during a period from November 2016 to April 2017. Then, proceed with bacterial cultivation and identification, antimicrobial susceptibility testing by broth microdilution and genetic pattern analysis using pulsed field gel electrophoresis (PFGE).
Results - Overall, bacterial cultivation was identified to B. pseudomallei 23.3% (93/400). The specimens which collected from carcasses of pigs slaughtered in Nakhon Pathom province detected 26.0% (52/200), while in Ratchaburi province was isolated from carcasses of pigs 20.8% (40/192) and goat 100% (1/1), but not detected B. pseudomallei from beef cattles (0/7). Gross lesion which observed B. pseudomallei infection was abscesses 40.7% (81/199), inflammation of capsule 7.4% (9/121), congestion 4.2% (1/24), white foci 2.7% (1/37) and pyometra 100% (1/1). In specimens containing abscesses, B. pseudomallei was detected from liver 40.4% (36/89), lung 23.8% (10/42), spleen 53.2% (33/62) and lymph node 66.7% (2/3). Antimicrobial susceptibility testing was found hightly susceptible 100% (89/89) to ceftazidime and imipenem followed by amoxicillin-clavulanic acid 98.9% (88/89), doxycycline 92.1% (82/89) and tetracycline 91.0% (81/89) but the effectiveness of trimethoprim-sulfamethoxazole was 34.8% (31/89). While, the genetic pattern analysis showed the DNA fingerprinting 46 different patterns. The strains isolated from specimens that collected from slaughterhouses in Nakhon Pathom province were presented 31 patterns and Ratchaburi province 17 patterns.
Conclusion - The findings showed that it has B. pseudomallei infected animals included in healthy animals slaughtered in slaughterhouses. The isolated strains were common detected from spleen liver and lung which contained abscesses, these presented highly genetic diversity. Therefore, best practices in biosafety are essential for stakeholders.
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References
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