Malondialdehyde levels in Canine Appendicular Osteosarcoma

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Arayaporn Macotpet
Ekkachai Pattarapanwichien
Fanan Suksawat

Abstract

Objective: To compare serum malondialdehyde (MDA) levels between clinically healthy dogs and dogs suffering from spontaneous appendicular osteosarcoma.


Materials and Method: Blood samples were obtained from 18 healthy dogs and 18 dogs with spontaneous appendicular osteosarcoma at Veterinary Teaching Hospital, Khon Kaen University. The final diagnosis of appendicular osteosarcoma was determined from histopathological findings. MDA levels were measured by thiobarbituric acid reactive substance assay. Packed cell volume, hemoglobin, red blood cell count and white blood cell count were measured using an automatic analyzer. Platelet estimation was done under the oil immersion lens of a microscope. Creatinine and ALT were measured using an automatic blood chemistry. Data are presented as mean ± standard deviation. Parametric data were determined using the independent-sample T test. Values of P<0.01 were considered significant.   


Results: Serum MDA levels were significantly higher in the appendicular osteosarcoma group than in clinically healthy dogs (P<0.001). Mean MDA level was 10.64±1.98 μmol/L in the appendicular osteosarcoma group and 4.30±0.49 μmol/L in the clinically healthy group. Packed cell volume (34.16±6.23% vs 46.66±6.08%), hemoglobin (11.92±2.08 g% vs 15.93±2.15 g%) and red blood cells (5.55±1.70 (×1012/L) vs 8.35±1.04 (×1012/L)) were all significantly lower in cancer group than in control group. Platelet estimations were adequate in both groups. White blood cell count, creatinine and alanine aminotransferase levels were not significantly different between both groups.


Conclusions: This study sustains the assumption that oxidative stress is associated with osteosarcoma in dogs, as serum MDA levels were significantly higher in appendicular osteosarcoma-bearing dogs compared to clinically healthy dogs.

Article Details

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Research articles

References

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