Anti- Helicobacter pylori and probiotic activity of Lactobacillus Strains
คำสำคัญ:
แลคโตบาซิลลัส, Helicobacter pylori, โรคติดเชื้อ, โพรไบโอติกบทคัดย่อ
Lactobacillus has beneficial effect to human health. In this study Lactobacillus were isolated from infant feces and fermented food to investigate for their inhibitory effect against Helicobacter pylori growth. A total of 400 Lactobacillus isolates were obtained. They were gram-positive, bacilli and catalase-negative. All isolates were tested for their anti-H. pylori activity by agar spot method. The results demonstrated that 100 isolates displayed no inhibitory activities, 227 isolates showed weak inhibitory activities and 71 isolates showed moderate inhibitory activities. Interestingly, 2 Lactobacillus isolates demonstrated strong inhibitory activities. They have probiotic activity including tolerance to pH 2.0, 3.0 and 4.0, and1-4% bile salt condition. Base on 16S rRNA gene sequence analysis, The strains were identified as Lactobacillus paracasei subsp. paracasei. This study demonstrated that Lactobacillus strains isolated from Thailand sources, such as infant feces and fermented food have anti-H. pylori activity, which may used as probiotics for prevention and treatment against H. pylori
infection.
เอกสารอ้างอิง
