Effects of deferoxamine on the survival of the neuroblastoma SH-SY5Y cells and neuroimmune response in the BV-2 microglial cells

Authors

  • Supanee Chounchay
  • Nuanchan Chutabhakdikul

Keywords:

deferoxamine, cell viability, SH-SY5Y, BV-2 cell, IL-10

Abstract

Abstract
Microglial are the resident immune cells in the central nervous system (CNS). They release cytokines and chemokines associate with inflammation and consequently lead to the neurological diseases. Iron overload in the CNS is one factor that triggers neuroinflammation. Deferoxamine (DFO) is an iron chelator widely used for removing excessive iron to protect neurons from iron overload. However, a high dose of DFO can induce oxygen depletion and hypoxic damage of neurons. It is still not clear how DFO has an impact on survival and death of cell in the nervous system. Therefore, the objective of this study is to investigate the effects of DFO on cell viability of neuron and microglia cells. The neuroblastoma (SH-SY5Y) and Microglia (BV-2) cell lines were cultured in a completed medium containing DFO at 25, 50, and 100 µM for 24 to 48 hours. Then, the cell viability and the expression of anti-inflammatory cytokine IL-10 were measured. The results showed that after 24 hours of DFO treatment, the cell viability of both cells were significantly decreased as compared to the control. Although the SH-SY5Y cell viability still decreases after 48 hours of DFO treatment, there is a trend to increases of BV-2 cell viability together with a significant increase in the level of IL-10 expression. The finding suggested that DFO treatment, even at low dosage, can induce neuronal and microglial cell death. Furthermore, enhance microglial cell survival and IL-10 expression indicated that the microglia might play an anti-inflammatory role following the hypoxic injury. Our results suggest that DFO should be carefully prescribed to avoid the adverse effects of DFO on hypoxia-induced cell death, especially within 24 hours after drug treatment. Finally, microglia might be a novel therapeutic target for the treatment of neurological diseases related to chronic neuroinflammation.

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Published

2020-12-30

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Original Article (บทความวิจัย)