Anti-inflammatory activity of Salacia chinensis L. extracts through the inhibition of nitric oxide secretion by macrophages

Authors

  • Supaporn Khamchun
  • Chonticha tananchai

Keywords:

Salacia chinensis L, inflammation, nitric oxide, macrophages

Abstract

Abstract
Salacia chinensis L. is extensively used as a traditional herbal medicine in Thailand for the severity alleviation of several diseases involved with inflammation such as asthma, cancer and diabetes mellitus. Nevertheless, the underlying mechanisms in this inflammatory effect of Salacia chinensis L. are still unclear. In the present study, we thus aimed to evaluate the capacity of Salacia chinensis L. for an inhibition of inflammatory process via nitric oxide secretion from RAW264.7 macrophage cell line induced by lipopolysaccharide. The extracts of Salacia chinensis L. with methanol and distilled water were determined cytotoxicity and the reduction of nitric oxide secretion by trypan blue exclusion and Griess reaction assay, respectively. The results demonstrated that both of Salacia chinensis L. extracts were not toxic to macrophage cell line. Furthermore, all concentrations of these extracts (100, 200 and 400 µg/mL) significantly diminish macrophage cell line for the secretion of nitric oxide in a dose dependence manner (p<0.05). Our findings implied that the extracts of Salacia chinensis L. had an inhibitory capacity of inflammation through nitric oxide secretion. The active compounds of Salacia chinensis L. in this effect may be further investigated.

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Published

2020-12-30

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Original Article (บทความวิจัย)