Cysticidal efficacy of methanolic extract from Jatropha curcas on Acanthamoeba spp.


  • Pannawich Siriwechviriya Microbiology and Parasitology Division, School of Medical Science, University of Phayao
  • Nannaphat mooktharakhosa Microbiology and Parasitology Division, School of Medical Science, University of Phayao
  • Ornampai Japa Microbiology and Parasitology Division, School of Medical Science, University of Phayao


Acanthamoeba spp., Jatropha curcas, cysticidal, J. curcas root, cysticidal efficacy, methanolic extracts


Introduction: Acanthamoeba is an opportunistic protozoon causing granulomatous amoebic encephalitis and amoebic keratitis (AK). Humans can be infections with a serious symptom especially contact lens wearers and immunocompromised patients. This study aimed to evaluate the cysticidal efficacy of methanolic extract of Jatropha curcas on Acanthamoeba spp. Acanthamoeba cyst-containing suspension of 7 x 104 cysts/ml was treated with methanolic extract of J. curcas and 1% sodium hypochlorite for 1 to 24 hours to determine the minimal cysticidal concentration of each disinfectant. Inverted phase-contrast microscopy was used to observe the morphologic changes. The minimum concentration methanolic extract of J. curcas root for 12 and 24 hours were 400 and 300 µg/ml, respectively. And, lethal concentration values which causes the death of 50% for 12 and 24 hours were 303 and 245 µg/ml respectively. The results show that, methanolic extract of J. curcas root can make a damage to Acanthamoeba cyst.


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