Evaluation of reticulocyte number counted by Coulter HmX® compared to Coulter STKS® and manual methods

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Published: Jan 1, 2009
Keywords:
Reticulocyte Coulter HmX® Coulter STKS® manual method

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Prapai Hemhorm
1 Division of Clinical Microscopy, Faculty of Medical Technology, Huachiew Chalermprakiet University, Thailand 2 Medical Technology Student, Faculty of Medical Technology, Huachiew Chalermprakiet University, Thailand
Surasak Wanrum
1 Division of Clinical Microscopy, Faculty of Medical Technology, Huachiew Chalermprakiet University, Thailand 2 Medical Technology Student, Faculty of Medical Technology, Huachiew Chalermprakiet University, Thailand
Karn Karanun
1 Division of Clinical Microscopy, Faculty of Medical Technology, Huachiew Chalermprakiet University, Thailand 2 Medical Technology Student, Faculty of Medical Technology, Huachiew Chalermprakiet University, Thailand
Somjit Likit
1 Division of Clinical Microscopy, Faculty of Medical Technology, Huachiew Chalermprakiet University, Thailand 2 Medical Technology Student, Faculty of Medical Technology, Huachiew Chalermprakiet University, Thailand
Prapaporn Tumkaew
1 Division of Clinical Microscopy, Faculty of Medical Technology, Huachiew Chalermprakiet University, Thailand 2 Medical Technology Student, Faculty of Medical Technology, Huachiew Chalermprakiet University, Thailand
Janjuree Kaewma
1 Division of Clinical Microscopy, Faculty of Medical Technology, Huachiew Chalermprakiet University, Thailand 2 Medical Technology Student, Faculty of Medical Technology, Huachiew Chalermprakiet University, Thailand
Yutthana Pengjam
1 Division of Clinical Microscopy, Faculty of Medical Technology, Huachiew Chalermprakiet University, Thailand 2 Medical Technology Student, Faculty of Medical Technology, Huachiew Chalermprakiet University, Thailand

Abstract

Number of reticulocyte can indicate efficiency of bone marrow on erythropoiesis. It also aids in diagnosis and follow up anemic patients. In addition, it can be used for determination of new red blood cell production after bone marrow transplantation. In this study, we evaluated reticulocyte number counted by Coulter HmX®, Coulter STKS automate cell counters and manual method. One hundred and fifty - five samples were studied. The reticulocyte counts were found to be 1.71±1.69 % (0.6–2.8%), 1.99±1.77% (0.7–2.8%) and 1.73±1.68% (0.5–2.0%) for Coulter STKS, Coulter HmX® and manual method, respectively. The reticulocyte number enumerated by these three techniques was statistically comparable (p>0.05). The correlation coefficient (r) of reticulocyte count between Coulter STKS® and Coulter HmX® was 0.975, Coulter STKS® and manual method was 0.995 and Coulter HmX® and manual method was 0.985. It was concluded that all these methods can be used in any laboratories for the reticulocyte count. Bull Chiang Mai Assoc Med Sci 2009; 42: 18-24.

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How to Cite
Hemhorm, P., Wanrum, S., Karanun, K., Likit, S., Tumkaew, P., Kaewma, J., & Pengjam, Y. (2009). Evaluation of reticulocyte number counted by Coulter HmX® compared to Coulter STKS® and manual methods. Journal of Associated Medical Sciences, 42(1), 19. Retrieved from https://he01.tci-thaijo.org/index.php/bulletinAMS/article/view/60092
Issue
Vol. 42 No. 1 (2009): January-April
Section
Research Articles

Personal views expressed by the contributors in their articles are not necessarily those of the Journal of Associated Medical Sciences, Faculty of Associated Medical Sciences, Chiang Mai University.