Antibacterial and antifungal activities Fak Khaao leaf extract

Abstract

     Fak Khaao, Thai local plant name was used for dietary as well as Thai traditional medicine. It has been shown to have antimicrobial activity, anticancer, antioxidant agents and antidiabetes. The aims of this work were to study antibacterial and antifungal activities of Fak khaao leave extract in order to develop leave extract preparation for skin treatment. Fak khaao leave was extracted by 4 different media including hexane, ethyl acetate, ethanol and methanol. Antimicrobial activity was studied by agar well diffusion method and agar dilution method. The result showed that hexane extract has no effect on bacteria (Staphylococcus aureus, Steptococcus pyogenes and Escherichia coli) or fungi (Candida albicans, Aspergillus fl avus, Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum gypseum, Epidermophyton fl occosum) tested. Methanol extract, though had no effect on bacteria or some fungi           (C. albicans and A. fl avus), but signifi cantly inhibited 4 pathogenic fungi tested (T. rubrum, T. mentagrophytes, M. gypseum E. floccosum, MIC= 78.125-312.5 mg/mL: zone size 12.2±2.1 mm -15.0±0.5 mm). However ethanol and ethyl acetate extracts showed antibacterial activity on gram positive bacteria tested (S. aureus, S. pyogenes) and antifungal activity against 4 pathogenic fungi tested (T. rubrum, T. mentagrophytes, M. gypseum E. floccosum). The antifungal activity (MIC= 156.2-312.5 mg/mL with zone size 12.0±0.5-24.0±1.5 mm) was more active than antibacterial activity (MIC= 625.0 mg/ml with zone size 12.0 ± 0.5 – 14.0±0.5 mm.)