Antibacterial Activity of Mitragyna Speciosa Korth. Extract on Streptococcus mutans
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Abstract
Objective: The purpose of this study aimed to evaluate the antibacterial effect of Mitragyna speciosa Korth. (Kratom) extract on Streptococcus mutans.
Materials and Methods: Crude extracts of kratom leaves were prepared using four extraction methods: maceration with 95% ethanol, maceration with 50% ethanol, decoction, and squeezing. S. mutans cultured in Mueller Hinton Broth was plated on Mitis Salivarius Agar. Paper discs containing kratom extracts from the four different extraction methods, and 0.12% chlorhexidine (as a positive control) were placed on agar plates. The inhibition zones were measured in millimeters, with clear zones indicating bacterial growth inhibition. The experiments were performed in triplicate. Data were recorded numerically and supplemented with images for analysis.
Results: The experiments indicated a similar clear zone area in shape and size. A clear inhibition zone was seen around the filter disc soaked in 0.12% chlorhexidine, indicating its antibacterial activity against S. mutans, while discs soaked in other extracts allowed normal bacterial growth. Red and white kratom extracts were unable to inhibit bacterial growth regardless of the extraction method used (maceration with 95% or 50% ethanol, decoction, or squeezing). However, reduced clear zones were observed around paper discs soaked with extracts from white kratom (squeezing method) and red kratom (maceration with 95% ethanol), which exhibited a slight tendency to inhibit bacterial growth.
Conclusion: Despite previous reports of antibacterial properties, kratom extracts obtained by the extraction methods in this study probably had a slight or no effect on S. mutans. Further investigation will provide more information on the effect of kratom on S. mutans.
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บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการลงตีพิมพ์ในวิทยาสารทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่นถือเป็นลิขสิทธิ์เฉพาะของคณะทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่น หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือเพื่อกระทำการใด ๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษร จากคณะทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่นก่อนเท่านั้น
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