Antioxidant Activity of Chlorhexidine in Various Concentrations and Effect of Chlorhexidine on Tooth Bond Strength with Resin Composite after Non–Vital Tooth Bleaching
Article Sidebar
Main Article Content
Abstract
The aim of this study was to compare the antioxidant activity between chlorhexidine in various concentrations and sodium ascorbate and to compare dentin bond strength between composite resin and dentin surface treated by chlorhexidine or sodium ascorbate solution after non-vital tooth bleaching. The solution of 20%, 10%, 5%, 2.5% and 1.25% chlorhexidine and 10% sodium ascorbate were prepared to analyse antioxidant activity by DPPH assay. Two concentrations of chlorhexidine with the highest antioxidant activity were selected to perform this study on surface treatment and shear bond strength test. Sixty extracted human upper premolar teeth were divided randomly into 4 groups (N=15): no surface treatment (negative control: C1); surface treatment with sodium ascorbate (positive control: C2); surface treatment with 20% chlorhexidine: T1 and 10% chlorhexidine: T2. Specimens were sectioned with slow-speed diamond disc. Dentin surface were prepared and bleached with sodium perborate for 7 days 3 cycles, surface treated by cotton pallet moistened with each solution for 5 mins, rinse off and immediately restored with two-step self-etch and composite resin in the cylinder with 4 mm height and diameter. The shear bond strength test was performed. Median Antioxidant Activity (AA%) by DPPH Assay of 20%, 10%, 5%, 2.5%, 1.25% chlorhexidine and 10% sodium ascorbate solution group were 33.44 (23.20,43.14), 20.80 (19.23,30.47), 23.98 (11.84,28.60), 10.40 (9.53,14.03), 15.86 (6.46,17.65) and 37.96 (34.54,53.28) % respectively. Median SBS in C1, C2, T1 and T2 group were 22.07 (18.14,25.08), 72.69 (67.56,81.67), 6.14 (2.91,8.41) and 14.30 (12.42,23.13) MPa respectively. Data was statistically analyzed by Kruskal-Wallis test and Pairwise Comparison. There were no significant differences of antioxidant activity between chlorhexidine and sodium ascorbate solution (p>0.05) except between sodium ascorbate solution group and 2.5% chlorhexidine group. There were significant differences of shear bond strength between both control group and experimental group (p<0.05) except between C1 group and T2 group. In summary, the antioxidant activity of highly concentrated chlorhexidine was not different from sodium ascorbate solution but effected to decrease dentin shear bond strength of composite resin after non-vital tooth bleaching.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการลงตีพิมพ์ในวิทยาสารทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่นถือเป็นลิขสิทธิ์เฉพาะของคณะทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่น หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือเพื่อกระทำการใด ๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษร จากคณะทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่นก่อนเท่านั้น
References
MacIsaac AM, Hoen CM. Intracoronal bleaching: concerns and considerations. J Can Dent Assoc 1994;60(1):57-64.
Attin T, Hannig C, Wiegand A, Attin R. Effect of bleaching on restorative materials and restorations--a systematic review. Dent Mater 2004;20(9):852-61.
Madison S, Walton R. Cervical root resorption following bleaching of endodontically treated teeth. J Endod 1990;16(12):570-74.
Jung KH, Seon EM, Choi AN, Kwon YH, Son SA, Park JK. Time of application of sodium ascorbate on bonding to bleached dentin. Scanning 2017;2017: 6074253.
Muraguchi K, Shigenobu S, Suzuki S, Tanaka T. Improvement of bonding to bleached bovine tooth surfaces by ascorbic acid treatment. Dent Mater J 2007;26(6):875-81.
Shinohara MS, Peris AR, Pimenta LA, Ambrosano GM. Shear bond strength evaluation of composite resin on enamel and dentin after nonvital bleaching. J Esthet Restor Dent 2005;17(1):22-9.
Topcu FT, Erdemir U, Ozel E, Tiryaki M, Oktay EA, Yildiz E. Influence of bleaching regimen and time elapsed on microtensile bond strength of resin composite to enamel. Contemp Clin Dent 2017;8(1):451-58.
Harrison MS, Wang Y, Frick KJ, Moniz J, Walker MP. Effects of alpha-tocopherol antioxidant on dentin-composite microtensile bond strength after sodium perborate bleaching. J Endod 2019;45(8):1053-59.
Garcia EJ, Oldoni TL, Alencar SM, Reis A, Loguercio AD, Grande RH. Antioxidant activity by DPPH assay of potential solutions to be applied on bleached teeth. Braz Dent J 2012;23(1):22-7.
Yeung SY, Huang CS, Chan CP, et al. Antioxidant and pro-oxidant properties of chlorhexidine and its interaction with calcium hydroxide solutions. Int Endod J 2007;40(11):837-44.
Scaffa PM, Vidal CM, Barros N, Gesteira TF, Carmona AK, Breschi L, et al. Chlorhexidine inhibits the activity of dental cysteine cathepsins. J Dent Res 2012;91(4):420-25.
Carrilho MR, Geraldeli S, Tay F, de Goes MF, Carvalho RM, Tjäderhane L, et al. In vivo preservation of the hybrid layer by chlorhexidine. J Dent Res 2007;86(6):529-33.
Shafiei F, Fekrazad R, Kiomarsi N, Shafiei E. Bond strength of two resin cements to dentin after disinfection pretreatment: effects of Er,Cr:YSGG laser compared with chemical antibacterial agent. Photomed Laser Surg 2013;31(5):206-11.
Briso ALF, Rahal V, Sundfeld RH, Santos PHd, Alexandre RS. Effect of sodium ascorbate on dentin bonding after two bleaching techniques. Oper Dent 2014;39(2):195-203.
Trindade T, Moura L, Raucci-Neto W, Messias D, Colucci V. Bonding effectiveness of universal adhesive to intracoronal bleached dentin treated with sodium ascorbate. Braz Dent J 2016;27(3):303-08.
Nishitani Y, Hosaka K, Hoshika T, Yoshiyama M, Pashley DH. Effects of chlorhexidine in self-etching adhesive: 24 hours results. Dent Mater J 2013;32(3):420-4.
Breschi L, Mazzoni A, Nato F, Carrilho M, Visintini E, Tjäderhane L, et al. Chlorhexidine stabilizes the adhesive interface: a 2-year in vitro study. Dent Mate 2010;26(4):320-25.
Stanislawczuk R, Reis A, Loguercio AD. A 2-year in vitro evaluation of a chlorhexidine-containing acid on the durability of resin-dentin interfaces. J Dent 2011;39(1):40-7.
Ricci HA, Sanabe ME, de Souza Costa CA, Pashley DH, Hebling J. Chlorhexidine increases the longevity of in vivo resin-dentin bonds. Eur J Oral Sci 2010;118(4):411-16.
Sacramento PA, de Castilho AR, Banzi EC, Puppi-Rontani RM. Influence of cavity disinfectant and adhesive systems on the bonding procedure in demineralized dentin-a one-year in vitro evaluation. J Adhes Dent 2012;14(6):575-83.
Mobarak EH, El-Korashy DI, Pashley DH. Effect of chlorhexidine concentrations on micro-shear bond strength of self-etch adhesive to normal and caries-affected dentin. Am J Dent 2010; 23(4):217-22.
Dalli M, Ercan E, Zorba YO, Ince B, Sahbaz C, Bahsi E, et al. Effect of 1% chlorhexidine gel on the bonding strength to dentin. J Dent Sci 2010;5(1):8-13.
Hamdan-Nassar T, Bellot-Arcis C, Paredes-Gallardo V, et al. Effect of 2% chlorhexidine following acid etching on microtensile bond strength of resin restorations: a meta-analysis. Medicina (Kaunas) 2019;55(12):769.
Loguercio A, Sr R, Polli L, Costa J, Michel M, Reis A. Influence of chlorhexidine digluconate concentration and application time on resin bond strength durability. Eur J Orol Sci 2009; 117(5):587-96.
Carrilho MR, Carvalho RM, Sousa EN, Nicolau J, Breschi L, Mazzoni A, et al. Substantivity of chlorhexidine to human dentin. Dent Mater 2010;26(8):779-85.
Misra DN. Interaction of chlorhexidine digluconate with and adsorption of chlorhexidine on hydroxyapatite. J Biomed Mater Res 1994; 28(11):1375-81.
Freitas C, Diniz H, Gomes J, Sinisterra R, Cortés M. Evaluation of the substantivity of chlorhexidine in association with sodium fluoride in vitro. Pesqui Odontol Bras 2003;17(1):78-81.
Hipólito V, Salvio L, Martins A, Goes M. Hybridization quality and bond strength of adhesive systems according to interaction with dentin. Eur J Dent 2013;7(3):315-26.
Zhou J, Tan J, Chen L, Li D, Tan Y. The incorporation of chlorhexidine in a two-step self-etching adhesive preserves dentin bond in vitro. J Dent 2009;37(10):807-12.
Van Landuyt KL, Snauwaert J, De Munck J, Peumans M, Yoshida Y, Poitevin A, et al. Systematic review of the chemical composition of contemporary dental adhesives. Biomaterials 2007;28(26):3757-85.