The Effects of Gnetum Gnemon Extract on Streptococcus Mutans, in Vitro Study

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Nattapon Rotpenpian
Suwanna Jitpukdeebodintra


Streptococcus mutans is a bacteria involved in the pathogenesis of dental caries. This study aimed to investigate Gnetum gnemon extract’s ability on bacterial viability, growth inhibition, acid production, glucosyltransferase enzyme formation, and the ability of adhesion of Streptococcus mutans ATCC 25175, in vitro. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of Gnetum gnemon extract were determined by a broth microdilution method. Inhibition of acid production, glucosyltransferase enzyme activity, and adhesion were determined. The results showed that the minimum inhibitory concentration of Gnetum gnemon extract was 15 mg/mL. The minimum bactericidal concentration was 30 mg/mL. At the minimum inhibitory concentration level, Gnetum gnemon extract showed an inhibitory effect on adhesion. However, Gnetum gnemon extract has no effect on acid production and glucosyltransferase enzyme formation of Streptococcus mutans ATCC 25175. These findings suggested that the Gnetum gnemon extract can inhibit the growth and adhesion of Streptococcus mutans ATCC 25175, in vitro, which causes dental caries in humans.


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Rotpenpian N, Jitpukdeebodintra S. The Effects of Gnetum Gnemon Extract on Streptococcus Mutans, in Vitro Study. KDJ [Internet]. 2021Aug.16 [cited 2021Oct.24];24(2):1-. Available from:
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