Cytotoxicity of Thai Propolis Crude Extracts on Human Periodontal ligament cell, In Vitro.
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Abstract
The aim of this study was to compare the cytotoxicity of Thai Propolis crude extracts in which PDL cells were stored in different concentrations and amount of time. Frozen PDL cells derived from extracted human premolars for orthodontic treatment were stored in each Thai Propolis extract concentrations (0.3125, 0.625, 1.25, 2.5, 5 mg/mL), serum free media as a negative control, and hydrogen peroxide as a positive control for 3, 6 and 12 hours. The number of PDL cell viability was assessed by PrestoBlue assay. The result showed that the lower concentration of Thai Propolis extract was more effective in maintaining the viability of PDL cells. In conclusion, Thai Propolis extract is not cytotoxic to PDL cells.
Article Details
บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการลงตีพิมพ์ในวิทยาสารทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่นถือเป็นลิขสิทธิ์เฉพาะของคณะทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่น หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือเพื่อกระทำการใด ๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษร จากคณะทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่นก่อนเท่านั้น
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