Cytotoxicity of Thai Propolis Crude Extracts on Human Periodontal ligament cell, In Vitro.

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Thalerngsak Samaksamarn
Chantida Pawaputanon Na Mahasarakham
Nutthapong Kantrong
Siripa Kamtawee
Nattapong Pannak
Chonlaya Suknim
Pattama Chailertvanitkul


The aim of this study was to compare the cytotoxicity of Thai Propolis crude extracts in which PDL cells were stored in different concentrations and amount of time. Frozen PDL cells derived from extracted human premolars for orthodontic treatment were stored in each Thai Propolis extract concentrations (0.3125, 0.625, 1.25, 2.5, 5 mg/mL), serum free media as a negative control, and hydrogen peroxide as a positive control for 3, 6 and 12 hours. The number of PDL cell viability was assessed by PrestoBlue assay. The result showed that the lower concentration of Thai Propolis extract was more effective in maintaining the viability of PDL cells. In conclusion, Thai Propolis extract is not cytotoxic to PDL cells.

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Samaksamarn T, Pawaputanon Na Mahasarakham C, Kantrong N, Kamtawee S, Pannak N, Suknim C, Chailertvanitkul P. Cytotoxicity of Thai Propolis Crude Extracts on Human Periodontal ligament cell, In Vitro. Khon Kaen Dent J [Internet]. 2021 Mar. 28 [cited 2024 Jun. 24];24(1):52-65. Available from:
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