Inhibitory Effects of Lemongrass Oil on Multispecies Subgingival Biofilm Formation in Vitro
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Abstract
Cymbopogon citratus (lemongrass) oil is a volatile oil obtained from the lemongrass leaves. It was reported to exhibit antibacterial activity towards periodontal pathogens, such as Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. However, there is no report on inhibitory effects of lemongrass oil (LG) against multispecies subgingival biofilm formation. The purpose of this study was to investigate the inhibitory effects of LG on subgingival biofilm formation using an in vitro model. Subgingival plaque samples from 5 periodontitis patients were cultivated in saliva-coated 96-well microtiter plates in the presence of LG at concentrations 1 - 6 µL/mL under anaerobic atmosphere at 37°C for 2, 4 and 8 days by replacing every 2 days with fresh medium alone or LG-containing medium. Quantification of biofilm formation was determined by staining with crystal violet. TaqMan-based real-time polymerase chain reaction was used to quantify A. actinomycetemcomitans and total bacteria in subgingival biofilm. The results showed that LG exhibited more than 80% anti-biofilm activity at 6 µL/mL. Moreover, LG at concentration 6 µL/mL was able to kill all A. actinomycetemcomitans in subgingival biofilm on day 2, 4, and 8. These results revealed that LG is able to inhibit in vitro subgingival biofilm formation and reduce the numbers of A. actinomycetemcomitans in multispecies subgingival biofilm.
Article Details
บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการลงตีพิมพ์ในวิทยาสารทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่นถือเป็นลิขสิทธิ์เฉพาะของคณะทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่น หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือเพื่อกระทำการใด ๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษร จากคณะทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่นก่อนเท่านั้น
References
Petersen PE, Ogawa H. Strengthening the prevention of periodontal disease: the WHO approach. J Periodontol 2005;76(12):2187-93.
Hoiby N, Bjarnsholt T, Givskov M, Molin S, Ciofu O. Antibiotic resistance of bacterial biofilms. Int J Antimicrob Agents 2010;35(4):322-32.
Socransky SS, Haffajee AD.Periodontal microbial ecology. Periodontol 2000 2005;38:135-87.
Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL, Jr. Microbial complexes in subgingival plaque. J Clin Periodontol 1998;25(2):134-44.
Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL, Jr. Microbial complexes in subgingival plaque. J Clin Periodontol 1998;25(2):134-44.
Pradhan-Palikhe P, Mantyla P, Paju S, Buhlin K, Persson GR, Nieminen MS, et al. Subgingival bacterial burden in relation to clinical and radiographic periodontal parameters. J Periodontol 2013;84(12):1809-17.
Wara-aswapati N, Pitiphat W, Chanchaimongkon L, Taweechaisupapong S, Boch JA, Ishikawa I. Red bacterial complex is associated with the severity of chronic periodontitis in a Thai population. Oral Dis 2009;15(5): 354-9.
Fine DH, Markowitz K, Furgang D, Fairlie K, Ferrandiz J, Nasri C, et al. Aggregatibacter actinomycetemcomitans and its relationship to initiation of localized aggressive periodontitis: longitudinal cohort study of initially healthy adolescents. J Clin Microbiol 2007;45(12):3859-69.
Zambon JJ. Periodontal diseases: microbial factors. Ann Periodontol 1996;1(1):879-925.
Slots J, Ting M. Actinobacillus actinomy cetemcomitans and Porphyromonas gingivalis in human periodontal disease: occurrence and treatment. Periodontol 2000 1999;20:82-121.
Torrungruang K, Jitpakdeebordin S, Charatkulangkun O, Gleebbua Y. Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Treponema denticola/ Prevotella intermedia Co-Infection Are Associated with Severe Periodontitis in a Thai Population. Plos One 2015;10(8).
Hyvarinen K, Laitinen S, Paju S, Hakala A, Suominen-Taipale L, Skurnik M, et al. Detection and quantification of five major periodontal pathogens by single copy gene-based real-time PCR. Innate Immun 2009;15(4):195-204.
Paju S, Pussinen PJ, Suominen-Taipale L, Hyvonen M, Knuuttila M, Kononen E. Detection of Multiple Pathogenic Species in Saliva Is Associated with Periodontal Infection in Adults. J Clin Microbiol 2009;47(1):235-8.
Ardila CM, Granada MI, Guzman IC. Antibiotic resistance of subgingival species in chronic periodontitis patients. J Periodontal Res 2010;45(4):557-63.
Rams TE, Degener JE, van Winkelhoff AJ. Antibiotic resistance in human chronic periodontitis microbiota. J Periodontol 2014;85(1):160-9.
Aiemsaard J, Aiumlamai S, Aromdee C, Taweechaisupapong S, Khunkitti W. The effect of lemongrass oil and its major components on clinical isolate mastitis pathogens and their mechanisms of action on Staphylococcus aureus DMST 4745. Res Vet Sci 2011;91(3):e31-7.
Satthanakul P, Taweechaisupapong S, Paphangkorakit J, Pesee M, Timabut P, Khunkitti W. Antimicrobial effect of lemongrass oil against oral malodour micro-organisms and the pilot study of safety and efficacy of lemongrass mouthrinse on oral malodour. J Appl Microbiol 2014; 118(1):11-7.
Lertsatitthanakorn P, Taweechaisupapong S, Aromdee C, Khunkitti W. In vitro bioactivities of essential oils for acne control. Int J Aromather 2006;16(1):43-9.
Taweechaisupapong S, Aieamsaard J, Chitropas P, Khunkitti W. Inhibitory effect of lemongrass oil and its major constituents on Candida biofilm and germ tube formation. S Afr J Bot 2012;81:95-102.
Taweechaisupapong S, Ngaonee P, Patsuk P, Pitiphat W, Khunkitti W. Antibiofilm activity and post antifungal effect of lemongrass oil on clinical Candida dubliniensis isolate. S Afr J Bot 2012;78:37-43.
Aiemsaard J, Aiumlamai S, Taweechaisupapong S, Aromdee C, Khunkitti W. Chemical composition, antioxidant activity and antibacterial action of eight essential oils against clinical isolates of mastitis pathogens. Int J Essential oil Ther 2010;4:37-43.
Bard M, Albrecht MR, Gupta N, Guynn CJ, Stillwell W. Geraniol interferes with membrane functions in strains of Candida and Saccharomyces. Lipids 1988;23(6):534-8.
Braga PC, Dal Sasso M.Morphostructural alterations of Candida albicans induced by thymol. A scanning electron microscopy study. J Chemother 2005;17:109.
Walker C, Sedlacek MJ. An in vitro biofilm model of subgingival plaque.Oral Microbiol Immunol 2007;22(3): 152-61.
Ruangcharoen S, Suwannarong W, Lachica M, Bolscher JGM, Nazmi K, Khunkitti W, et al. Killing activity of LFchimera on periodontopathic bacteria and multispecies oral biofilm formation in vitro. World J Microbiol Biotechnol 2017;33(9):167.
Taweechaisupapong S, Pinsuwan W, Suwannarong W, Kukhetpitakwong R, Luengpailin S. Effects of Streblus asper leaf extract on the biofilm formation of subgingival pathogens. . S Afr J Bot 2014;94:1-5.
Ashimoto A, Chen C, Bakker I, Slots J. Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. Oral Microbiol Immunol 1996; 11(4):266-73.
Sedlacek MJ, Walker C. Antibiotic resistance in an in vitro subgingival biofilm model. Oral Microbiol Immunol 2007;22(5):333-9.
Nadkarni MA, Martin FE, Jacques NA, Hunter N. Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set. Microbiology 2002;148(1):257-66.
Morillo JM, Lau L, Sanz M, Herrera D, Martin C, Silva A. Quantitative real-time polymerase chain reaction based on single copy gene sequence for detection of periodontal pathogens.J Clin Periodontol 2004;31(12):1054-60.
Lewis K. Riddle of biofilm resistance. Antimicrob Agents Chemother 2001;45(4):999-1007.
Donlan RM, Costerton JW. Biofilms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev 2002;15(2):167-93.
Schwering M, Song J, Louie M, Turner RJ, Ceri H. Multi-species biofilms defined from drinking water microorganisms provide increased protection against chlorine disinfection. Biofouling 2013;29(8):917–28.
van der Veen S, Abee T. Mixed-species biofilms of Listeria monocytogenes and Lactobacillus plantarum show enhanced resistance to benzalkonium chloride and peracetic acid. Intl J Food Microbiol 2011;144(3):421–31.
Wang R, Kalchayanand N, Schmidt JW, Harhay DM. Mixed biofilm formation by Shiga toxin-producing Escherichia coli and Salmonella enterica serovar Typhimurium enhanced bacterial resistance to sanitization due to extracellular polymeric substances.J Food Prot 2013;76(9):1513–22.
Anwar H, Strap JL, Chen K, Costerton JW. Dynamic interactions of biofilms of mucoid Pseudomonas aeruginosa with tobramycin and piperacillin. Antimicrob Agents Chemother 1992;36(6):1208-14.
Amorena B, Gracia E, Monzon M, Leiva J, Oteiza C, Perez M, et al. Antibiotic susceptibility assay for Staphylococcus aureus in biofilms developed in vitro. J Antimicrob Chemother 1999;44(1):43-55.