Effect of Hydroxyl Radical from Blue Light and Hydrogen Peroxide on Porphyromonas gingivalis in Biofilms
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Abstract
Periodontal diseases are inflammatory diseases caused by groups of selected bacteria such as Porphyromonas gingivalis. These bacteria contain black pigment which is sensitive to blue light (BL). The previous studies have shown that hydroxyl radical (-OH) produced from BL and hydrogen peroxide (H2O2) can cause cellular damage and inhibited this bacterium. Therefore, this study evaluates the use of BL and H2O2 in single treatment and combination on the survival of P. gingivalis in biofilms. The -OH produced from BL and H2O2 were also studied by using the fluorescence probe technique. P. gingivalis biofilms were prepared from a subject
with chronic periodontitis. BL source laser with the wavelength of 405 nm and power density of 50 mW/cm2 was used as BL treatment. 1% H2O2 (v/v) was used in the H2O2 treatment. The survival fractions of P. gingivalis were determined at 0, 3 and 6 h post-treatment. This study found that treatment with H2O2 alone showed the greatest inhibitory effect against P. Gingivalis with an immediate effect while BL alone and BL+H2O2 took 6 hour post-treatment to inhibit P. gingivalis.
Article Details
บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการลงตีพิมพ์ในวิทยาสารทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่นถือเป็นลิขสิทธิ์เฉพาะของคณะทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่น หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือเพื่อกระทำการใด ๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษร จากคณะทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่นก่อนเท่านั้น
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