Collection Efficacies of Double Dose Platelet by Blood Cell Separators

Abstract

ABSTRACT: Background: New generation blood cell separators enable multiple unit collection of platelets from the one eligible donor in a single procedure. The advantages of this procedure are to maximize donor resources and platelet inventory as well asto minimize the possibilities of transfusion associated diseases and production costs. Objectives: The aim of this study was to evaluate the collection efficacy of two blood cell separator, the Amicus 3.1(AM) and the Trima Accel 5.0 (TA) for double dose platelet. In vitro platelet quality and in vivo platelet transfusion effectiveness were also studied. In addition donor safety and satisfaction were observed. Method: 45 eligible donors were collected for double dose platelets for the target of 6.5x10¹¹ by using two blood cell separators and with interval of 4 week. The products were measured for platelet yield, residual white blood cell (WBC), pH, and volume. Platelets were transfused to patients and transfusion effectiveness was measured by the corrected count increments 1 hour (CCI-1) and 24 hour (CCI-24) after transfusion. Results: The Amicus produced a significantly higher platelet yield with lower residual WBC counts than that of the Trima Accel (7.24x10¹¹ vs. 6.85x10¹¹, p<0.001; 0.34 vs. 0.82x10⁶ WBCs per unit, p=0.022). For leukoreduction, all platelet units (splitted single unit) had fewer than 5x10⁶ WBCs per unit. The mean amounts of whole blood processed with the Amicus were significant higher than that of the Trima Accel (3574.09 vs. 3355.67 ml, p<0.001). The Amicus and the Trima Accel were insignificantly different in regard to processing time, collection efficiency, platelet volume, and anticoagulant used. No severe donor reactions were observed during or after the procedure. Only a few differences in donor satisfaction were found between the two systems. The Amicus was more preferable by donors than the Trima Accel, especially regarding the use of the pressure cuff, which facilitates the draw cycles. Conclusions: The effectiveness of double dose platelet collection and the quality of platelet product in vitro by new generation blood cell separators has been revealed by this study. . Double dose plateletpheresis was performed efficiently and safely from both cell separators. All the platelet units pass international standard requirement. The donor safety was also observed.