Evaluation of IMUGARD III-PL (4P) for Platelet Concentrates

Abstract

Abstract: This study was carried out to evaluate the ectiveness of IMUGARD III-PL (4P) to remove leukocytes from single donor platelet concentrate (PC) using 24 IMUGARD II-PL (4P) for laboratony use for 24 units of single donor PC. The fitrations were done within 12 hours affer pration and storage at 22'C. The fittions were pormed in closed system. Technicon H3 RTC was used for platelet and white blood cell (WBC) counts for pre and post-flltroducts except for post-fitration, WBC were counled by using Nageotte Brite Line Chamber. The results indicated that prefilitration platelets x 10¹¹/bag ranged from 2.71-5.51 with mean = 3.87 and SD = 0.72, while post-filtration platets x 10¹¹/bag ranged from 2.38-4.92 with mean = 3.39 and SD = 0.67. The percant yield of PC ranged from 77.68 - 93 with mean = 87.58 and SD = 4.40. WBC x 107bag in pre-fittration PC were ranged from 2-320.4 with mean = 73.27 and
SD = 81.52, while post-fitration WBC x 10/bag ranged from 0-0.035 with mean = 0.01 and SD = 0.02. The percent removal of WBC ranged from 99.69-100 with the fitration times ranged from 8 to 11 min/bag. Conclusion: IMUGARD II-PL (4P) is highly effective for leukoftration from single donor PC which is comparable with standard "third ganeration" set. The residual leukocytes were <=0.035x10⁶/bag. Transfusion of PC with WBC to less than 5x10⁶ , non hemoldic transion reaction, CMV transmission and HLA alloimmunization can be reduced.