ธาลัสซีเมียและภาวะขาดเหล็กในกลุ่มประชากรที่ให้ผลบวกต่อการตราจกรอง OF Test และ KKU-DCIP-Clear

Abstract

Abstract: Screenind for thalassemua and abnomal hemoglobin using an OF test and KKU-DCIP clear
were carried out in 192 nornal adult dung their routine medical check up at the faculty of Asso-
ciated Medical Sciences Khon Kaen University. One hundred and nine (56.7%) were positive
for either one or both tests. In these positive samples, complete hematological profile and molecular
defects were examined. These included hemoalobin electrophoresis and quantitation detection of
Ol-thalassemia 1 and Ol-thalassemia 2 gene deletion using PCR and Southern blot, idention of
aplha con sp and B-thalassemia gene using ASPCR and determination of serum ferritin level. Of the 109
positive samples 52.3% could be classified as HbE, 11.3% were alpha-thalassemia 1, 21.3% were alpha-
thalassemia 2, 28% were B-thalassemia, 8.5% were iron deficiency, 5.7% were
iron depleted. Some positive sample were accompanied by more than one thalassemia genes
whereas some were thalassemias with iron deficiency. All sample with HbE were positive on KKU-
DCIP clear test but two non HbE samples (1.8%) were false positive. Neither thalassemia gene nor
serum ferritin abnormality was observed in 22 cases (20 2%) positive for OF test alone. This data
indicates the need for improvement of the OF test in order to reduce a number of false positive
sample and obtain more specific results in thalassemia screening program.