Detection of Red Cell Antibodies by Enzyme Technique

Authors

  • Kanchana Outrakoolpoonsuk Department of Transfusion Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Sasitorn Bejrachandra Department of Transfusion Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Jariya Saipin Department of Transfusion Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Wipanee Leehaphaiboonsakun Department of Transfusion Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Varaporn Suratanarungsun Department of Transfusion Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Pisanupong Plubjuice Department of Transfusion Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University

Keywords:

Red cell antibodies, Enzyme

Abstract

Abstract: In a retrospective study on blood samples from 28,708 patients requing blood transfusion at Siriraj Hospital during October 1997 to September 19998, 1,044 samples (3.6%) were found to be positive for antibody screening. Antibody identification was perforned using various techniques including enzyme. Specific antibodies were identitied in 860 samples (82.4%) 263 samples (30 6%) of these antibodies were detected by enzyme only. Nonspecific and unidentified antibodies were found in 141 case (13.5%) and 43 cases (4.1%) respectively in the study. Among these groups, 70 samples (49.6%) of nonspecific antibodies and 43 samples (4.1%) of unidentied antibodies were detected. Furthermore, 64.3% of Lewis antibodies and 85% of Rh antibodies were detected by
enzyme only.

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References

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ทัศน์ยานี จันทนยิ่งยง. เวชศาสตร์การธนาคารเลือด. กรุงเทพฯ: ธรรกมลการพิมพ์, 2541:86,130.

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Published

2018-12-30

Issue

Vol. 9 No. 2: April-June 1999

Section

นิพนธ์ต้นฉบับ (Original article)

License

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