Reticulocyte Analysis by Coulter Gen S
Keywords:
Reticulocyte count, AutomationAbstract
Abstract: The reticulocyte count is useful for measuring of erythropoietic activity, anemia diagnosis, monitoring of therapy or as a way of checking early regeneration after marrow or stem cell trantation. We evaluated the counting of reticulocytes in peripheral blood with newly developed automated hematology analyzer. Coulter Gen S and compared the results with manual method. Venous
blood was obtained from 45 healthy individuals with normal hematologic data and 70 anemic patients. Results showed that there was no statistical difference between both methods and a good correlation with = 0,900 was observed. The automated method is precise and accurate and also provide other reticulocyte parameters including mean reticulocyte volume (MRV), immature reticu-
locyte fraction (18F), percent and absolute count of high light scatter reticulocyte (HILR) which can be used for evaluation of reticulocyte maturity.
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References
Savage RA, Skoog DP, Rabinovitch A. Analytical inaccuracy and imprecision in reticulocyte counting. A preliminary report form the College of American Pathologists reticulocyte project. Blood Cell 1985,11:97-112.
Koepke JF, Koepke JA. Reticulocytes. Clin Lab Haematol 1986;8:169-79.
Lohmann RC, Crawford LN, Wood DE. Proficiency testing in reticulocyte counting. Clin Lab Haematol 1994;16:57-64.
Heilmeyer L. Blutfarbstoffwechselstudien. Dtsch Arch Klin Med 1931;171:123-53.
Peebles DA, Hochberg A, Clarke TD. Analysis of manual reticulocyte counting. Am J Clin Pathol 1981;76:713-7.
NCCLS. Proposed standard H16-P. Method for reticulocyte counting. Villanova, PA: National Committee for Clinical Laboratory Standards, 1985.
Coulter Corp. Coulter Gen S System on line reference manual. Coulter Corp. PN 4237221, 1996.
Hall R, Malia RG. Basic haematological practice. In: Hall R, Malia RG, eds. Medical laboratory Haematology. 2'm ed. Oxford: Butterworth-Heinemann, 1991:84-112.
Charuruks N. Limpanasithikul W. Voravud N, Virochpoka T. Nuchprayoon C. Reference ranges of reticulocytes in adults. J Med Assoc Thai 1998;81:357-64.
Khuhapinant A, Bunyaratvej A, Tatsumi N, Pribwia M, Fucharoen S. Number and maturation of reticulocytes in various genotypes of thalassemia as assessed by flow cytometry. Acta Haematol 1994;91:119-25-25
Lamchiagdhase P, Pattanapunyasat K. Tangtiyaphan W, Kulsrisombat K. Flow cytometric reticulocyte analysis using thiazole orange. J Med Tech Assoc Thai 1996;24:157-66.
Disse A, Kurth D. Circulating reticulocytes in normal adults as determined by the new methylene blue method. Am J Clin Pathol 1970;53:481-4.
Carter JM, McSweeney PA, Wakem PJ, Nemet AM. Counting reticulocytes by flow cytometry: use of thiazole orange. Clin Lab Haematol 1989;11:267-71.
Ferguson DJ, Lee S, Gordon PA. Evaluation of reticulocyte counts by flow cytometry in a routine laboratory. Am J Clin Hematol 1990;33:13-7.
Bowen D, Bentley N, Hoy T, Cavill I. Comparison of a modified thiazole orange techique with a fully automated analyser for reticulocyte counting. J Clin Pathol 1991;44:130-3.
Lofness KG, Kohnke ML, Geier NA. Evaluation of automated reticulocyte counts and their reliability in the presence of Howell-Jolly bodies. Am J Clin Pathol 1994;101:85-90.
Tatsumi N, Tsuda I, Kojima K, Nari M, Setoguchi K.
An automated reticulocyte counting method: preliminary observations. Med Lab Sci 1989;46:157-60.
Takubo T, Kitano K, Ohto Y, Ikemoto T, Kikuchi T, Inai S. The usefulness of the R-1000 automated reticulocyte counter in a clinical hospital laboratory. Eur J Haematol 1989;43:88-9.
Tarallo P, Humbert JC, Mahassen P, Fournier B, Henny J. Reticulocytes: Biological variations and reference limits. Eur J Haematol 1994;53:11-5.
Gardner FH, Nathan DG, Piomelli S, Cummins JF. The erythrocythaemic effects of androgen. Br J Haematol 1968;14:611-5.
Buttarello M, Bulian P, De Pra M, Barbera P, Rizzotti P. Reticulocyte quantification by Coulter MAXM VCS (volume, conductivity, light scatter) technology. Clin Chem 1996;42:1930-7.
Rudensky B. Comparison of a semi-automated new Coulter methylene blue method with fluorescence flow cytometry in reticulocyte counting. Scand J Clin Lab Invest 1997;57:291-6.
Davies JI, Smyth MS, Martin JHJ. Automated reticulocyte counting revaluation of the CoulterR STKS haematology analyser reticulocyte counting function. Clin Lab Haematol 1997:19:89-92
Lowenstein LM. The mammalian reticulocyte. Int Rev Cytol 1959;8:135-74.
d'Onofrio G, Chirillo R, Zini G, et al. Simultaneous measurement of reticulocyte and red blood cell indices in healthy subjects and patients with microcytic and macrocytic anemia. Blood 1995;85:818-23
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