Determination of Screening Coagulogram by Automated Analyzer
Keywords:
Prothrombin time, Activated partial thromboplastin time, Thrombin time, Automation, Tilt-tube methodAbstract
Abstract: Prothrombin time (PT), activated partial thromboplastin time (APIT), and thrombin time (TT) are common screening laboratory tests that are useful in the diagnosis of coagulation disorders and monitoring anticoagulant therapy. Recently, the automated machines have been introduced to measure the clotting end points of the screening tests above. Usually, the manufacturers of some of these instruments have advocated the use of special brands of commercial reagents. The purpose
of this study was to compare the three kinds of commercial reagents for PT, APTT, and TT by fully automated BCT system with manual tilt-tube method in 80 healthy subjects. Almost all of the test results from the automated analyzer were significantly shorter than manual method. On the basis of these data, it is recommended that the laboratory should set the own reference range for the
screening coagulation tests due to different kind of sensitivity of the reagents and the method used in measuring the clotting times.
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References
National Committee for Clinical Laboratory Standards. Procedures for the collection of diagnostic blood specimens by venipuncture. 3rd ed. Approved Standard, NCCLS document H3-A3. Villanova, Pa:NCCLS; 1991.
National Committee for Clinical Laboratory Standards. Collection, transport, and processing of blood specimens for coagulation testing and performance of coagulation assays. 2nd ed. Approved Guideline, NCCLS document H21-A2. Villanova, Pa: NCCLS; 1991.
Yawn BP, Loge C, Dale J. Prothrombin time. One tube or two. Am J Clin Pathol 1996,105:794-7.
Brigden ML, Graydon C. McLeod B, Lesperance M. Prothrombin time determination. The lack of need for a discard tube and 24-hour stability. Am J Clin Pathol 1997;108:422-6.
Gottfried EL, Adachi MM. Prothrombin time and activated partial thromboplastin time can be performed on the first tube. Am J Clin Pathol 1997;107:681-3.
McGlasson DL, More L, Best HA, Norris WL, Doe RH, Ray H. Drawing specimens for coagulation testing : is a second tube necessary? Clin Lab Sci 1999:137-9.
Ivey LM, Thom JY, Baker RI. Single or duplicate analysis for automated prothrombin time and activated partial thromboplastin time? Pathol 1997;29:67-71.
Behringwerke AG. Behring Coagulation Timer instruction manual. Version 1.4. Frankfurt, Behring Diagnostics. 1996.
Poller L, Thomson JM, Yee KF. Automated versus manual techniques for the prothrombin time test: results of proficiency assessment studies. Br J Haematol 1978;38:391-9.
Starr H, Rhoades P, Lam Po Tang PRLC, Archer GT. Prothrombin time: an evaluation for four thromboplastins and four machines. Pathol 1986;12:567-74.
Zucker S, Brosious E, Cooper GR. One-stage prothrombin time survey. Am J Clin Pathol 1970;53 : 340-7.
D'Angelo A, Seveso MP, D'Angelo SV, et al. Comparison of two automated coagulometers and the manual tilt-tube method for the determination of prothrombin time. Am J Clin Pathol 1989;92:321-8.
Cunningham MT, Johnson GF, Pennell BJ, Olson JD. The reliability of manufacturer-determined, instrument-specific International Sensitivity Index values for calculating the International Normalized Ratio. J Clin Pathol 1994;102:128-33.
Howarth S. Activated partial thromboplastin time reagents : an evaluation. Br J Biomed Sci 1993;50:109-13.
Turi DC, Peerschke El. Sensitivity of three activated partial thromboplastin time reagents to coagulation factor deficiencies. Am J Clin Pathol 1986;85:43-9.
Rosborough TK. Comparing different lots of activated partial thromboplastin time reagent. Analysis of two methods. Am J Clin Pathol 1998;110:173-7.
Koepke JA. Partial thromboplastin time test. Proposed performance guidelines. ICSH Panel on the PTT. Thromb Haemost 1986;55:143-4.
Kritikos VG, Melhem MF. Comparison of three prothrombin time and activated partial thromboplastin time reagent systems on the MLA 700 coagulation analyzer. Clin Lab Sci 1993;6:302-7.
Van der Velde EA, Poller L. The APTT monitoring of heparin. The ISTH/ICSH collaborative study. Thromb Haemost 1995;73:73-81.
Opartkiattikul N. Standardization of coagulation tests. Southeast Asian J Trop Med Public Health 1999:30 (suppl 3):79-85.
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