Detection of Platelet Antibodies by Platelet Suspension Immunofluorescent Technique

Abstract

Abstract: There is an urgent need for a simple and reliable method for the detection of platelet antibodies, comparable to the antiglobulin test in red cell serology, for better investigation, following up and monitoring the diseases in patients suffering from bleeding disorder effected from thrombocytopenia. In order to develop and demonstrate that platelet susbension immunoftuorescent test (PSIFT' is sufficient for the detection of platelet-reactive alloantibodies, 84 serum sample of patients suffering from hematologic malignancies, idiopathic thrombocytopenic purpura (ITP) with platelet transfusion therapy from the Maharaj Nakorn Chiang Mai Hospital were tested. The optimal condition of FTC-conjugated rabbit anti-human immunoolobulins was titrated and the result was 1:30 and 25 (L for dilution and volume, respectively. In addition, the specilicity, sensitivity and reproducibility were also studied. It was shown that all results were acceptable. The detection of anti-human platelet antibodies in serum of patients with diseases mentioned above resulted in 48.8% positive. To exclude that such those were not antibodies specific to HLA class I molecules, lymphocytotoxicity test was performed. The results showed they were all negative. This result indicated that antibodies were specific to platelet membrane antigens. Furthermore, the posive serum were determined to specify whether there was anti-platelet glycoprotein IIb/Ila and/or platelet specific antigens by an indirect ElISA. It was found that they were all significanty positive.