The Quality and Stability of Normal Pooled Plasma for Coagulation Test

Abstract

Abstract: The quality and stability of nomal pooled plasma (NP) were tested by activated partial thromboplastin time (APTI) and assay factor VIII clotting activity (FVIII:C), using commercial calibration plasma as a standard plasma. NP was prepared from 5 males and 5 females who have normal coagulogram. NP was kept in aliquots at -15'C,-30 Cand-70'C by nomal freezing and snap
freezing. These samples were tested APTTT and FVIIC at 1, 2,4, 6, 8, 10 and weeks. After 12 weeks the coefficient of variation (CV) of APTT -15'C 30 Cand-70'C by normal freezing is 142, 5.0 and 4.2 percent respectively and by snap freezing technic CV is 11.4, 6.4 and 3.3 percent respectively. The CV of FVIIICat-15'C, 30 Cand-70'C by normal freezing and snap frezing are more than 10 percent. The conclusion of this study found that home made NP kept at -30'C and -70'C by normal and snap freezing is suitable to use as normal control for APTT since CV is less than 10 percent. This NP can be kept 12 weeks at both -30 Cand -70'C. However, NP is not good to be standard plasma for FVIII:C assay since CV is changed more than 10 percent.