The Use of Acid Phosphatase Cytochemical Staining for Detection of 'I cell Leukemia in Children; Compare to Immunophenotyping by Monoclonal Antibodies Using Flow Cytometry

Authors

  • Gavivann Veerakul Division of Hematology/Oncology, Department of Pediatrics, Faculty of Medicine Siriraj Hospital
  • Kalaya Techawanich Division of Hematology/Oncology, Department of Pediatrics, Faculty of Medicine Siriraj Hospital
  • Yodsaward Theptaradol Division of Hematology/Oncology, Department of Pediatrics, Faculty of Medicine Siriraj Hospital
  • Kleebsabai Sanpakit Division of Hematology/Oncology, Department of Pediatrics, Faculty of Medicine Siriraj Hospital

Keywords:

Acid phosphatase, T cell leukemia, Immunophenotype

Abstract

Abstract: The use of cytochemical staining with Acid Phosphatase for diagnosis of T cell leukemia in children were statistically analysed, and compared to the results from immunophenotypic study, using T cell monoclonal antibodies by flow cytometry. Results: The use of acid phosphatase staining for T cell leukemia showed 0.909 sensitivity (95%CI 0.764, 0.969) and 0.942 specificity (95%CI 0.906, 0.965). Among the 4 monoclonal antibodies (CD7, CD2, CD3, CD5) used for T cell leukemia, the CD7
had the highest sensitivity of 1.000 (95%CI 0.906, 1.000) and CD5 had the highest specificity of 0.997 (95%CI 0.977, 0.999). Conclusion: Acid phosphatase staining can be used to differentiate T cell leukemia from other types of leukemia in places where immunophenotypic and cytogenetic studies were unavailable. Immunophenotyping was still the standard investigation, recommended in classification of childhood leukemias,and the diagnosis of T cell leukemia especially CD7 and CD5.

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Published

2018-12-30

Issue

Vol. 12 No. 4: October-December 2002

Section

นิพนธ์ต้นฉบับ (Original article)

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