Study of the Effect of Blood Storage on CD59 Expression of Blood Cells
Keywords:
Blood storage, CD59 expressionAbstract
Abstract : The objective of this study was to explore the ettect of blood storage and ditterent anticoagulants on flow-cytometric analysis of CD59 expression on erythrocytes and granulocytes. Heparinized blood samples were used for analysis of CD59 expression on both erythrocytes and granulocytes, while EDTA blood was only appropriate for analysis of erythrocytes. Blood samples were collected from 10 healthy persons, 10 patients with aplastic anemia (AA), 10 patients with myelodysplastic syndrome
(MDS), 10 patients with lymphoma and 13 patients with paroxysmal nocturnal hemoglobinuria (PNH). Analyses of CD59 expression were performed at 0, 24, 48 hours after blood samples were collected and kept at room temperature. The CD59 expression as indicated by the proportions (%) of CD59 positive cells were not significantly different (p>0.05) at different time interval of analyses. However, at 48 hours of storage, the proportions of CD59 positive granulocytes from a few blood samples were unacceptably deviated (>1%) from these analysed within 24 hours. The CD59 expressions on erythrocytes from EDTA and heparinized blood were not signifcantly different (p>0.05). In conclusion, for analyses of CD59 expression on erythrocytes, both EDT'A and heparin can be used as anticoagulants and blood samples should be sent to operating laboratories within 48 hours. For CD59 expression on granulocytes, heparinized blood should be used and analysed within 24 hours of collection.
Downloads
References
Hall C, Richards SJ, Hillman P. The Glycosylphosphathidylinosital Anchor and Paroxysmal Nocturnal Haemoglobinuria/Aplasia Model. Acta Haematol 2002;108:219-30.
Luzzatto L. Paroxysmal Nocturnal Hemoglobinuria. International Society of Haematology 1996:120-7.
Oelschlaegel U, Besson I, Arnoulet C, et al. A standardized flow cytometric method for screening paroxysmal nocturnal hemoglobinuria(PNH) measuring CD55 and CD59 expression on erythrocytes and granulocytes. Clin Lab Haem 2001;23:81-90.
Kinoshita T. Overview of Paroxysmal Nocturnal Hemoglobinuria: Molecular Genetics. In : Omine M, Kinoshita T, eds. Paroxysmal Noctumal Hemoglobinuria and Related Disorders. Springer, 2001:3-7.
Hall SE, Rosse WF. The use of monoclonal antibodies and flow cytometry in the diagnosis of paroxysmal nocturnal hemoglobinuria. Blood 1996;87:5332-40.
Meletis J, Terpos E, Samarkos M, et al. Detection of CD55 and/or CD59 Deficient Red Cell Populations in Patients with Aplastic Anemia and Myelodysplastic Syndrome. Leukemia and Lymphoma 1999.
Luzzatto L, Hillmen P. Laboratory methods used in the investigation of paroxysmal nocturnal hemoglobinuria (PNH). In : Dacie JV, Lewis SM, eds. Practical Haematology. 8" ed. Churchill Livingstone 1995.
Nishimura J, Hirota T, Kanakura Y, et al. Long-term support of hematopoiesis by a single stem cell clone in patients with paroxysmal nocturnal hemoglobinuria. Blood 2002:99:2748-51.
Terpos E, Michali E, Meletis Chr, et al. "PNH-LIKE" Red Cell Populations in Patients with Anemia of Different Etiology. Oslo 1998.
วันชัย วนะชิวนาวิน, เกรียงศักดิ์ ภักดีสุวรรณ. Paroxysmal Nocturnal Hemoglobinuria. วารสารโลหิตวิทยาและเวชศาสตร์บริการโลหิต 1995;5:54-62.
Meletis J, Terpos E.Recent insights into the pathophysiology of paroxysmal nocturnal hemoglobinuria. Med Sci Monit, 2003;9:RA161-72.
Nathalang O, Chuansumrit A, Prayoonwiwat W. Rapid screening of PNH red cell populations using the gel test. Southeast Asian J Trop Med Public Health 2003;34:887-90.
Richards SJ, Rawstron AC and Hillmen P. Application of flow cytometry to the Diagnosis of Paroxysmal Nocturnal Hemoglobinuria. Cytometry 2000;42:223-33.
Pakdeesuwan K, Wanachiwanawin W, Siripanyapinyo U, Pattanapanyasat K, Wilairat P, Issaragrisil S. Immunophenotypic discrepancies between granulocyte and erythroid lineages in peripheral blood of patients with paroxysmal nocturnal haemoglobinuria. European Journal of Haematology 2000;65:8-16.
จารุวรรณ โชคคณาพิทักษ์. การเปรียบเทียบค่าเฉลี่ยของประชากร 2 กลุ่ม. ใน: อรุณ จิรวัฒนกุล, บรรณาธิการ. ชีวสถิติ. ภาควิชาชีวสถิติและประชากรศาสตร์ คณะสาธารณสุขศาสตร์ มหาวิทยาลัยขอนแก่น, 2542:144-59.
Vittorio R. The molecular basis of paroxysmal nocturnal hemoglobinuria. Haematologica 2000;85:82-7.
Parker CJ. Molecular Basis of Paroxysmal Nocturnal Hemoglobinuria. Stem cell 1996;14:396-411.
Wanachiwanawin W, Piyawattanasakul N, Visuthisakchai S, Issaragrisil S. Nature of PNH Clones in Aplastic Anemia. In : Omine M, Kinoshita T, eds. Paroxysmal Nocturnal Hemoglobinuria and Related Disorders. Springer 2001:229-33.
Borowitz MJ. Flow Cytometry Testing in PNH. How Much is Enough? Cytometry 2000;42:221-2.
สุรพล เกาะเรียนอุดม, กิตติพงศ์ รุ่งเรืองธนะกิจ, วัชระ กสิณฤกษ์, ชัยยศ คุณานุสนธิ์, โกวิทย์ พัฒปัญญาสัตย์. การบริหารคุณภาพโฟลไซโทเมทรี: การวิเคราะห์หาประชากรย่อยของเซลล์ลิมโฟไซต์. กรุงเทพฯ: ชมรมโฟลไซโทเมทรีแห่งประเทศไทย, 2541:68-9.
Pakdeesuwan K, Wilairat P, Wanachiwanawin W. Paroxysmal nocturnal hemoglobinuria (PNH) : From Basic Research to Clinical Points of View. Mahidol Journal 1997;4:29-34.
Dunn DE, Tanawattanacharoen P, Boccuni P, et al. Paroxysmal nocturnal hemoglobinuria cells in patients with bone marrow failure syndromes. Annal Int Med 1999131:401-8.
Nakao S, Wang H, Chuhjo T. Clinical Significance of Increase PNH-Type Cells in the Peripheral Blood of Patients with Aplastic Anemia and Refractory Anemia. In : Omine M, Kinoshita T, eds. Paroxysmal Nocturnal Hemoglobinuria and Related Disorders. Springer, 2001:129-37.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
