Pre-transplant factors influencing HLA crossmatch in deceased donor kidney transplantation
Keywords:
HLA crossmatch, Deceased donors, Kidney transplantationAbstract
Abstract:
Introduction: Factor affecting the waiting list for deceased donor kidney transplantation is the retrieval time of the donor’s organs until a transplant kidney has been given to the patient. Hence, the delivery of the spleen and/or lymph nodes to the laboratory must be kept in the transport medium at the appropriate temperature. Besides, the HLA crossmatch testing time is another factor affecting kidney transplantation. Objective: To study retrospective information about factors affecting the HLA crossmatch test for kidney transplantation from deceased donors. Materials and Methods: The numbers of kidneys that could not be transplanted and the duration
of the HLA crossmatch test using complement-dependent cytotoxicity (CDC) and CDC-Antihuman globulin (CDC-AHG) for kidney transplantation from deceased donors to the patient candidates on waiting list of the Organ Donation Centre and the National Blood Centre, Thai Red Cross Society, to receive a kidney transplant were studied. Results: The total number of deceased donors was 1,233 cases with an upward trend every year. Fourteen samples from the deceased donors (1.13%) could not be used for HLA crossmatch test, owing to cell low viability (n = 13, 1.05%) and cell insufficiency for testing (n = 1, 0.08%). Moreover, the duration of testing time was approximately 5 to 6 hours, starting from obtaining the spleen and/or lymph nodes of the deceased donors to the time that the laboratory reported the results to the Organ Donation Centre. Conclusion: The important factors affecting the HLA crossmatch time before deceased donor kidney transplantation including not only specimen transportation but also the quality of laboratory management would be beneficial to support the increasing number of deceased kidney donors.
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References
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