The effect of adding platelet-rich plasma to fibrin glue on release of platelet growth factors and its stability
PGF and stability of fibrin glue with platelet
Keywords:
Platelet growth factors, Platelet-rich plasma, Fibrin glueAbstract
Background: Fibrin glue is a commonly used hemostatic agent.
Objective: The aim of the study was to determine the effect of adding platelets to fibrin glue concerning the release of platelet growth factors (PGF) and its stability.
Methods: Platelet-rich plasma (PRP) was added to a fibrinogen solution and mixed with 250 IU/mL thrombin
solution to form fibrin glue. The contents in the completely formed glue were squeezed out at 5, 60, 120, and
300 minutes to determine PGF. Then the stability of fibrin glue with and without platelets in plasma, urine and saliva was studied.
Results: Fibrin glue with platelets showed increased amounts of platelet-derived growth
factor (PDGF)-AB and PDGF-BB by time-dependent fashion. The fibrin glue with platelets showed less reduced mass, reflecting better stability, compared with that without platelets in plasma, urine and saliva.
Conclusion: Adding PRP to the fibrin glue released PGF and enhanced its stability
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References
2. Martinowitz U, Saltz R. Fibrin sealant. Curr Opin Hematol. 1996;3:395-402.
3. Wagner W, Wiltfang J, Pistner H, Yildirim M, Ploder B, Chapman M, et al. Bone formation with a biphasic calcium phosphate combined with fibrin sealant in maxillary sinus floor elevation for delayed dental implant. Clin Oral Implants Res. 2012;23:1112-7.
4. Avanogmacr;lu A, Celik A, Ulman I, Ozcan C, Kavakli K, Nişli G. Safer circumcision in patients with haemophilia: the use of fibrin glue for local haemostasis. BJU Int. 1999;83:91-4.
5. Rodriguez-Merchan EC. Local fibrin glue and chitosan-based dressings in haemophilia surgery. Blood Coagul Fibrinolysis. 2012;23:473-6.
6. Marx G, Mou X. Characterizing fibrin glue performance as modulated by heparin, aprotinin, and factor XIII. J Lab Clin
Med. 2002;140:152-60.
7. Murphy WG, Milne AA, Ruckley CV, Kubba AK, Palmer KR. Fibrin sealant: update on clinical studies. Transfus Sci.1996;17:341-2.
8. Spotnitz WD. Fibrin Sealant: the only approved hemostat, sealant, and adhesive-a laboratory and clinical perspective. ISRN Surg. 2014;2014:203943.
9. Meyer-Ingold W, Eichner W. Platelet-derived growth factor. Cell Biol Int. 1995;19:389-98.
10. Gassling VL, Acil Y, Springer IN, Hubert N, Wiltfang J. Plateletrich plasma and platelet-rich fibrin in human cell culture. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;108:48-55.
11. He L, Lin Y, Hu X, Zhang Y, Wu H. A comparative study of platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) on the effect of proliferation and differentiation of rat osteoblasts in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;108:707-13.
12. Josh F, Tobita M, Tanaka R, Orbay H, Ogata K, Suzuki K, et al. Concentration of PDGF-AB, BB and TGF-β1 as valuable human serum parameters in adipose-derived stem cell proliferation. J Nippon Med Sch. 2013;80:140-7.
13. Lee JW, Kwon OH, Kim TK, Cho YK, Choi KY, Chung HY, et al. Platelet-rich plasma: quantitative assessment of growth factor levels and comparative analysis of activated and inactivated groups. Arch Plast Surg. 2013;40:530-5.
14. Su CY, Kuo YP, Tseng YH, Su CH, Burnouf T. In vitro release of growth factors from platelet-rich fibrin (PRF): a proposal to optimize the clinical applications of PRF. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009;108:56-61.
15. Rock G, Neurath D, Lu M, Alharbi A, Freedman M. The contribution of platelets in the production of cryoprecipitates for use in a fibrin glue. Vox Sang. 2006;91:252-5.
16. Martineau I, Lacoste E, Gagnon G. Effects of calcium and thrombin on growth factor release from platelet concentrates: kinetics and regulation of endothelial cell proliferation. Biomaterials. 2004;25:4489-502.
