Comparison of Xpert CT/NG assay with Gram’s staining and culture for identifying Neisseria gonorrhoeae
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Abstract
Gonorrhoea is one of the most important sexually transmitted diseases (STDs). There are high incidence in adolescents in Thailand. This disease is caused by the fastidious bacteria, called Neisseria gonorrhoeae. In these days, the molecular biological assay, Xpert CT/NG, has been used to diagnose N. gonorrhoeae from urine specimen. The objective of this study was to compare N. gonorrhoeae detection between the Xpert CT/NG assay and the conventional assays, Gram staining and culture. 81 patients got laboratory tests for diagnosis of gonorrhoea at STDs clinic of the Office of Disease Prevention and Control region 3, Nakhon Sawan from May 2020 to January 2021. The Xpert CT/NG assay, Gram staining and culture yielded 40 positive samples (49.4%, 95% CI 38.5% - 60.3%), 33 positive samples (40.7%, 95% CI 30.0% - 51.4%) and 27 positive samples (33.3%, 95% CI 23.0% - 43.6%), respectively. For the concordance between the Xpert CT/NG assay with Gram staining and culture, % agreement and kappa were 91.4%, 0.83 and 84.0, 0.68, respectively. The mean of the Xpert CT/NG turnaround time was 1.1 days. The advantages of the Xpert CT/NG assay were high detection rate of N. gonorrhoeae, rapid testing and easy sample collection. Because of the expensive cost, the Xpert CT/NG assay should be additional test of conventional assays to reduce false negative and increase more accurate results.
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