Comparison of Xpert CT/NG Assay with Gram Stain and Culture for Identifying Neisseria gonorrhoeae
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Abstract
Gonorrhoea is one of the most important sexually transmitted diseases (STDs). In Thailand, the incidence is high among adolescents. This disease is caused by the contagious bacterium Neisseria gonorrhoeae. Nowadays, the molecular biology test Xpert CT/NG is used to diagnose N. gonorrhoeae from urine samples. The aim of this study was to compare the detection of N. gonorrhoeae between the Xpert CT/NG assay and the conventional assays, Gram stain and culture. 81 patients were screened for gonorrhoea at the STD clinic of the Office of Disease Prevention and Control Region 3, Nakhon Sawan, between May 2020 and January 2021. The Xpert CT/NG assay, Gram stain and culture yielded 40 positive samples (49.4%, 95% CI 38.5% - 60.3%), 33 positive samples (40.7%, 95% CI 30.0% - 51.4%) and 27 positive samples (33.3%, 95% CI 23.0%-43.6%), respectively. For agreement between the Xpert CT /NG assay with Gram stain and culture, the percent agreement and kappa were 91.4%, 0.83 and 84.0%, 0.68, respectively. The average turnaround time of the Xpert CT /NG was 1.1 days. The advantages of the Xpert CT /NG test were the high detection rate of N. gonorrhoeae, the rapid test and the simple sample collection. Due to the high costs, the Xpert CT/NG assay should be an additional test to the conventional assays to reduce false negative results and increase the accuracy of the results.
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