Encapsulation of Rice Bran Oil in Solid Lipid Nanoparticles (SLN) for Skin Hydration and Viscoelasticity
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Abstract
The purposes of this study were to encapsulate rice bran oil (RBO) in solid lipid nanoparticles (SLN) by high pressure homogenization technique and to investigate the influence of formulation compositions (lipid, surfactant, and RBO concentrations) and process parameters (homogenization pressures and size reduction times) on physical properties of the SLN. The system was also tested in 10 healthy human volunteers for potential skin hydration and viscoelasticity benefits. Results showed that the optimized SLN formulation with particle size below 200 nm could be achieved by using Compritol® 888 ATO, RBO, and Pluronic® F68 at the concentration below 2.5, 2.5 and above 2.5%w/w, respectively.Homogenization pressure at 500 to 700 bars significantly reduced particle size from 500 to 160 nm. The SLN mean particle size (160±20 nm, polydispersity index<0.2) remained unchanged for at least 28 days at 25 ÌC. Skin hydration of cream containing RBO encapsulated in SLN (1.28 %w/w RBO, SLN/cream base ratio was 51.2:48.8 %weight) was significantly higher than the cream base after applying at 7, 14 and 28 days and the skin viscoelasticity was significantly increased in the ranges of 4-5% at 7, 14 and 28 days, respectively (p<0.05). In conclusion, SLN represents a promising delivery system of RBO for skin hydration and viscoelasticity.
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