Main Article Content
Alternanthera philoxeroides has been used as antipyretic, dressing for wounds and ulcers in Thai traditional medicine. The present study aims to investigate the immunomodulating activity of this plant by using splenocyte proliferation test. The results showed that methanol (MeOH) extract, diethyl ether (Et₂O) and n-butanol (n-BuOH) fractions of A. philoxeroides were not cytotoxic to host cells up to the concentration tested (200 µg/ml). For immunomodulating study, MeOH extract, Et₂O and n-BuOH fractions suppressed splenocyte proliferation. Et₂O fraction at a concentration of 50 µg/ml significantly suppressed splenocyte proliferation (p < 0.05). Whereas chikusetsusaponin IVa increased splenocyte proliferation in dose-dependent manner and at a concentration of 25 µg/ml the compound significantly increased splenocyte proliferation (p < 0.05).
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
In the case that some parts are used by others The author must Confirm that obtaining permission to use some of the original authors. And must attach evidence That the permission has been included
Brinkeborn RM, Shah DV, Degenring FH. 1999. Echinaforce and other echinacea fresh plant preparations in the treatment of the common cold. A randomized, placebo controlled, double-blind clinical trial. Phytomedicine 6: 1-6.
Fang J, Jia W, Gao W, et al. 2007. Antitumor constituents from Alternanthera philoxeroides. J Asian Nat Prod Res 9: 511-515.
Fang JB, Duan HQ, Zhang YW, et al. 2006. Chemical constituents from herb of Alternanthera philoxeroides. Zhongguo Zhong Yao Za Zhi31: 1072-1075.
Gohar AA, Maatooq GT, Niwa M, et al. 2002. A new triterpene saponin from Chenopodium ficifolium. Z Naturforsch [C] 57: 597-602.
Hikino H, Kiso Y, Kinouchi J, et al. 1985. Antihepatotoxic actions of ginsenosides from Panax ginseng roots1. Planta Med 51: 62-64.
Jiang WL, Luo XL, Kuang SJ. 2005. Effects of Alternanthera philoxeroides griseb against dengue virus in vitro. Di Yi Jun Yi Da Xue Xue Bao 25: 454-456.
Jiang WL, Yang ZQ, Chen W, et al. 2007. Effects of Alternanthera philoxeroides griseb against respiratory syncytial virus infection in mice. Nan Fang Yi Ke Da Xue Xue Bao 27: 62-64.
Kumar RA, Sridevi K, Kumar NV, et al. 2004. Anticancer and immunostimulatory compounds from Andrographis paniculata.J Ethnopharmacol 92: 291-295.
Lin TD, Kondo N, Shoji J. 1976. Studies on the constituents of Panacis japonici rhizoma. V.1 the structure of chikusetsusaponin I, Ia, Ib, IVa and glycoside p1. Chem Pharm Bull 24: 253-261.
Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 65: 55-63.
Nakamura A, Nagai K, Suzuki S, et al. 1986. A novel method of screening for immunomodulating substances, establishment of an assay system and its application to culture broths of microorganisms. J Antibiot (Tokyo) 39: 1148-1154.
Oda K, Matsuda H, Murakami T, et al. 2000. Adjuvant and haemolytic activities of 47 saponins derived from medicinal and food plants. Biol Chem 381: 67-74.
Puri A, Saxena R, Saxena RP, et al. 1993. Immunostimulant agents from Andrographis paniculata. J Nat Prod 56: 995-999.
Schreck C, Lamberson H, Davey F. 1982. Characterization of the B lymphocyte response to pokeweed mitogen. Ann Clin Lab Sci 12: 455-462.
Sun HX, Wang H. 2006. Immunosuppressive activity of the ethanol extract of Siegesbeckia orientalis on the immune responses to ovalbumin in mice. Chem Biodivers 3: 754-761.
Zhang SM, He YS, Tabba HD, et al. 1988. Inhibitor against the human immunodeficiency virus in aqueous extracts of Alternanthera philoxeroides. Chin Med J (Engl) 101: 861-866.
Zhang YB, Qin F, Sun HX. 2006. Immunosuppressive activity of Semen persicae ethanol extract on specific antibody and cellular response to ovalbumin in mice. Chem Biodivers 3: 967-974.