Development of Cleansing Toner from Puag-Haad

Abstract

Introduction: Puag-Haad is a bubble from aqueous crude extract prepared from Artocarpus lakoocha heartwood. Oxyresveratrol is a major constituent which possesses an antibacterial (especially Staphylococcus aureus) and antityrosinase activities. The main objective of this study was to develop cleansing toner containing Puag-Haad. Methods: TLCzdensitometric method was used to determine the amount of oxyresveratrol in PuagzHaad. Effect of Puag-Haad on inhibition of S. aureus was tested by broth macrodilution and spread plate. The cleansing toners containing Puag-Haad were formulated by varying co-solvents. Then, the developed formulations were studied including stability test using Heating-Cooling cycle, skin irritation, and satisfaction tests in healthy volunteers (n=30). Results: The result showed that a milligram of Puag-Haad contained 0.702±0.007 mg of oxyresveratrol. MBC value of Puag-Haad against S. aureus was 1.56 mg/ml. Then, the cleansing toners containing Puag-Haad (0.625% w/w) were developed. We found that the formulation No.16 which composed of glycerin 4%w/w, propylene glycol 4%w/w, and 70%sorbitol 2%w/w as a co-solvent, had a good physical stability including clear solution, adherence to skin, and good skin absorption. The formulation No. 16 with varied concentration of Puag-Haad (0.078, 0.156, 0.313, and 0.625%w/w namely formulation 16.1, 16.2, 16.3 and 16.4 respectively) were then formulated. The formulations no.16.2 and 16.3 had a good physical and chemical stability including no change of color, clear solution, no precipitant, and significantly unchanged of pH after Heating-Cooling cycles. Moreover, the developed formulations still showed antibacterial activity. After that, the skin irritation and satisfaction tests of developed cleansing toners were studied in healthy volunteers (n=30). Skin irritation was no reported. The satisfied test indicated that the cleansing toner of formulation 16.2 had higher satisfaction scores in color, odor, adherence to skin, and good skin absorption than those of formulation no.16.3. Conclusion: This study achieved to develop of cleansing toner containing Puag-Haad. The formulation No.