ฤทธิ์ต้านออกซิเดชันของสารสกัดบริสุทธิ์บางส่วน จากเปลือกมังคุด

Main Article Content

ณรงค์ พันหอม
วัชรีวรรณ มหามิตร
รติยา คูเขตพิทักษ์วงศ์

Abstract

The objective of this study was to screen antioxidant activity of partial purified extracts from mangosteen pericarp. Dried mangosteen pericarps were refluxed in methanol at 60°C for 6 h. Methanol extract was then separated by partitioning between dichloromethane and water. The dichloromethane phase was dried and partial purified fractions were obtained by silica gel column chromatography eluting with dichloromethane-methanol gradient. Thin-layer chromatography was carried out. Antioxidant activity of the fractions were studied using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) technique. Trolox was used as positive control. Twenty-eight fractions were obtained from the column chromatography. Only six fractions showed a spot of a-mangostin in TLC chromatograms. Concentrations of Trolox required to inhibit DPPH and ABTS radical formation by 50% (IC50) were 258.8±0.47 mM and 182.9±0.54 mM, respectively.  It was found that fractions No.1, 3 and 4 had less antioxidant activity (IC50 value > 1 mg/ml). There was no significant difference between fractions No.2, 5 and 6 in antioxidant activity (IC50 values ranged from 200-300 mg/ml). The three fractions would be considered to be used for preparation of a health product.

Downloads

Download data is not yet available.

Article Details

Section
Appendix